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Influence of osmolarity and hydration on the tear resistance of the human amniotic membrane


Bircher, Kevin; Merluzzi, Riccardo; Wahlsten, Adam; Spiess, Deborah; Simões-Wüst, Ana Paula; Ochsenbein-Kölble, Nicole; Zimmermann, Roland; Deprest, Jan; Mazza, Edoardo (2020). Influence of osmolarity and hydration on the tear resistance of the human amniotic membrane. Journal of Biomechanics, 98:109419.

Abstract

The amnion is considered to be the load-bearing part of the fetal membranes. We investigated the influence of osmolarity of the testing medium and hydration on its fracture toughness. Mode I fracture tests revealed that physiological variations in the bath osmolarity do not influence the tear resistance of amnion, while larger changes, i.e. from physiological saline solution to distilled water, lead to a significant reduction of the fracture toughness. Uniaxial tensile tests on collagen hydrogels confirmed the reduction in toughness, suggesting that lower bath osmolarity triggers changes in the failure properties of single collagen fibers. Prenatal surgeries, in particular fetoscopic procedures with partial amniotic carbon dioxide insufflation, might result in dehydration of the amnion. Dehydration induced a brittle behavior; however, subsequent rehydration for 15 min resulted in a similar tear resistance as for the fresh tissue.

Abstract

The amnion is considered to be the load-bearing part of the fetal membranes. We investigated the influence of osmolarity of the testing medium and hydration on its fracture toughness. Mode I fracture tests revealed that physiological variations in the bath osmolarity do not influence the tear resistance of amnion, while larger changes, i.e. from physiological saline solution to distilled water, lead to a significant reduction of the fracture toughness. Uniaxial tensile tests on collagen hydrogels confirmed the reduction in toughness, suggesting that lower bath osmolarity triggers changes in the failure properties of single collagen fibers. Prenatal surgeries, in particular fetoscopic procedures with partial amniotic carbon dioxide insufflation, might result in dehydration of the amnion. Dehydration induced a brittle behavior; however, subsequent rehydration for 15 min resulted in a similar tear resistance as for the fresh tissue.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Obstetrics
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Life Sciences > Biophysics
Health Sciences > Orthopedics and Sports Medicine
Physical Sciences > Biomedical Engineering
Health Sciences > Rehabilitation
Language:English
Date:2 January 2020
Deposited On:15 Jan 2021 09:43
Last Modified:04 Feb 2021 14:36
Publisher:Elsevier
ISSN:0021-9290
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.jbiomech.2019.109419
PubMed ID:31679754

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