Header

UZH-Logo

Maintenance Infos

Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions


Dumur, Tao; Duncan, Susan; Graumann, Katja; Desset, Sophie; Randall, Ricardo S; Scheid, Ortrun Mittelsten; Prodanov, Dimiter; Tatout, Christophe; Baroux, Célia (2019). Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions. Nucleus, 10(1):181-212.

Abstract

The eukaryotic cell nucleus is a central organelle whose architecture determines genome function at multiple levels. Deciphering nuclear organizing principles influencing cellular responses and identity is a timely challenge. Despite many similarities between plant and animal nuclei, plant nuclei present intriguing specificities. Complementary to molecular and biochemical approaches, 3D microscopy is indispensable for resolving nuclear architecture. However, novel solutions are required for capturing cell-specific, sub-nuclear and dynamic processes. We provide a pointer for utilising high-to-super-resolution microscopy and image processing to probe plant nuclear architecture in 3D at the best possible spatial and temporal resolution and at quantitative and cell-specific levels. High-end imaging and image-processing solutions allow the community now to transcend conventional practices and benefit from continuously improving approaches. These promise to deliver a comprehensive, 3D view of plant nuclear architecture and to capture spatial dynamics of the nuclear compartment in relation to cellular states and responses.

Abstract

The eukaryotic cell nucleus is a central organelle whose architecture determines genome function at multiple levels. Deciphering nuclear organizing principles influencing cellular responses and identity is a timely challenge. Despite many similarities between plant and animal nuclei, plant nuclei present intriguing specificities. Complementary to molecular and biochemical approaches, 3D microscopy is indispensable for resolving nuclear architecture. However, novel solutions are required for capturing cell-specific, sub-nuclear and dynamic processes. We provide a pointer for utilising high-to-super-resolution microscopy and image processing to probe plant nuclear architecture in 3D at the best possible spatial and temporal resolution and at quantitative and cell-specific levels. High-end imaging and image-processing solutions allow the community now to transcend conventional practices and benefit from continuously improving approaches. These promise to deliver a comprehensive, 3D view of plant nuclear architecture and to capture spatial dynamics of the nuclear compartment in relation to cellular states and responses.

Statistics

Citations

Dimensions.ai Metrics
10 citations in Web of Science®
8 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

2 downloads since deposited on 12 Jan 2021
2 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:580 Plants (Botany)
Scopus Subject Areas:Life Sciences > Cell Biology
Uncontrolled Keywords:Cell Biology
Language:English
Date:1 January 2019
Deposited On:12 Jan 2021 13:55
Last Modified:13 Jan 2021 21:01
Publisher:Taylor & Francis
ISSN:1949-1034
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1080/19491034.2019.1644592

Download

Gold Open Access

Download PDF  'Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions'.
Preview
Content: Published Version
Filetype: PDF
Size: 7MB
View at publisher
Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)