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Copper resistance genes of Burkholderia cenocepacia H111 identified by transposon sequencing


Higgins, Steven; Gualdi, Stefano; Pinto‐Carbó, Marta; Eberl, Leo (2020). Copper resistance genes of Burkholderia cenocepacia H111 identified by transposon sequencing. Environmental Microbiology Reports, 12(2):241-249.

Abstract

Copper is an essential element but in excess is highly toxic and therefore cytoplasmic levels must be tightly controlled. Member of the genus Burkholderia are highly resistant to various heavy metals and are often isolated from acidic soils where copper bioavailability is high. In this study, we employed transposon sequencing (Tn‐Seq) to identify copper resistance genes in Burkholderia cenocepacia H111. We identified a copper efflux system that shares similarities with the plasmid‐based copper detoxification systems found in Escherichia coli and Pseudomonas syringae. We also found that several of the identified resistance determinants are involved in maintaining the integrity of the cell envelope, suggesting that proteins located in the outer membrane and periplasmic space are particularly sensitive to copper stress. Given that several of the resistance genes are required for the repair and turnover of misfolded proteins, we suggest that copper toxicity is caused by protein damage rather than by oxidative stress.

Abstract

Copper is an essential element but in excess is highly toxic and therefore cytoplasmic levels must be tightly controlled. Member of the genus Burkholderia are highly resistant to various heavy metals and are often isolated from acidic soils where copper bioavailability is high. In this study, we employed transposon sequencing (Tn‐Seq) to identify copper resistance genes in Burkholderia cenocepacia H111. We identified a copper efflux system that shares similarities with the plasmid‐based copper detoxification systems found in Escherichia coli and Pseudomonas syringae. We also found that several of the identified resistance determinants are involved in maintaining the integrity of the cell envelope, suggesting that proteins located in the outer membrane and periplasmic space are particularly sensitive to copper stress. Given that several of the resistance genes are required for the repair and turnover of misfolded proteins, we suggest that copper toxicity is caused by protein damage rather than by oxidative stress.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:580 Plants (Botany)
Scopus Subject Areas:Life Sciences > Ecology, Evolution, Behavior and Systematics
Life Sciences > Agricultural and Biological Sciences (miscellaneous)
Uncontrolled Keywords:Agricultural and Biological Sciences (miscellaneous), Ecology, Evolution, Behavior and Systematics
Language:English
Date:1 April 2020
Deposited On:19 Jan 2021 16:19
Last Modified:30 Jan 2021 12:00
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN:1758-2229
OA Status:Closed
Publisher DOI:https://doi.org/10.1111/1758-2229.12828
Project Information:
  • : FunderSNSF
  • : Grant ID31003A_169307
  • : Project TitleCommunication in bacterial biofilms

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