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Analysis of serum glycome by lectin microarrays for prostate cancer patients - a search for aberrant glycoforms


Bertok, Tomas; Jane, Eduard; Chrenekova, Nikola; Hroncekova, Stefania; Bertokova, Aniko; Hires, Michal; Vikartovska, Alica; Kubanikova, Petra; Sokol, Roman; Fillo, Juraj; Kasak, Peter; Borsig, Lubor; Tkac, Jan (2020). Analysis of serum glycome by lectin microarrays for prostate cancer patients - a search for aberrant glycoforms. Glycoconjugate journal, 37(6):703-711.

Abstract

This is the first work focused on glycoprofiling of whole N- and O- glycome using lectins in an array format applied for analysis of serum samples from healthy individuals, benign prostate hyperplasia (BPH) patients, and prostate cancer (PCa) patients. Lectin microarray was prepared using traditional lectins with the incorporation of 2 recombinant bacterial lectins and 3 human lectins (17 lectins in total). Clinical validation of glycans as biomarkers was done in two studies: discrimination of healthy individuals with BPH patients vs. PCa patients (C vs. PCa) and discrimination of healthy individuals vs. BPH and PCa patients (H vs. PCond). Single lectins (17 lectins) and a combination of two lectins (136 binary lectin combinations) were applied in the clinical validation of glycan biomarkers providing 153 AUC values from ROC curves for both studies (C vs. PCa and H vs. PCond). Potential N- and O-glycans as biomarkers were identified and possible carriers of these glycans are shortly discussed.

Abstract

This is the first work focused on glycoprofiling of whole N- and O- glycome using lectins in an array format applied for analysis of serum samples from healthy individuals, benign prostate hyperplasia (BPH) patients, and prostate cancer (PCa) patients. Lectin microarray was prepared using traditional lectins with the incorporation of 2 recombinant bacterial lectins and 3 human lectins (17 lectins in total). Clinical validation of glycans as biomarkers was done in two studies: discrimination of healthy individuals with BPH patients vs. PCa patients (C vs. PCa) and discrimination of healthy individuals vs. BPH and PCa patients (H vs. PCond). Single lectins (17 lectins) and a combination of two lectins (136 binary lectin combinations) were applied in the clinical validation of glycan biomarkers providing 153 AUC values from ROC curves for both studies (C vs. PCa and H vs. PCond). Potential N- and O-glycans as biomarkers were identified and possible carriers of these glycans are shortly discussed.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > Biochemistry
Life Sciences > Molecular Biology
Life Sciences > Cell Biology
Language:English
Date:December 2020
Deposited On:21 Jan 2021 13:14
Last Modified:22 Jan 2021 21:01
Publisher:Springer
ISSN:0282-0080
Additional Information:This is a post-peer-review, pre-copyedit version of an article published in Glycoconjugate journal. The final authenticated version is available online at: https://doi.org/10.1007/s10719-020-09958-4
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/s10719-020-09958-4
PubMed ID:33119808

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Embargo till: 2021-12-31