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Quantification of mechanical forces and physiological processes involved in pollen tube growth using microfluidics and microrobotics


Burri, Jan T; Munglani, Gautam; Nelson, Bradley J; Grossniklaus, Ueli; Vogler, Hannes (2020). Quantification of mechanical forces and physiological processes involved in pollen tube growth using microfluidics and microrobotics. In: Geitmann, Anja. Pollen and Pollen Tube Biology : Methods and Protocols. Humana, New York: Springer, 275-292.

Abstract

Pollen tubes face many obstacles on their way to the ovule. They have to decide whether to navigate around cells or penetrate the cell wall and grow through it or even within it. Besides chemical sensing, which directs the pollen tubes on their path to the ovule, this involves mechanosensing to determine the optimal strategy in specific situations. Mechanical cues then need to be translated into physiological signals, which eventually lead to changes in the growth behavior of the pollen tube. To study these events, we have developed a system to directly quantify the forces involved in pollen tube navigation. We combined a lab-on-a-chip device with a microelectromechanical systems-based force sensor to mimic the pollen tube's journey from stigma to ovary in vitro. A force-sensing plate creates a mechanical obstacle for the pollen tube to either circumvent or attempt to penetrate while measuring the involved forces in real time. The change of growth behavior and intracellular signaling activities can be observed with a fluorescence microscope.

Abstract

Pollen tubes face many obstacles on their way to the ovule. They have to decide whether to navigate around cells or penetrate the cell wall and grow through it or even within it. Besides chemical sensing, which directs the pollen tubes on their path to the ovule, this involves mechanosensing to determine the optimal strategy in specific situations. Mechanical cues then need to be translated into physiological signals, which eventually lead to changes in the growth behavior of the pollen tube. To study these events, we have developed a system to directly quantify the forces involved in pollen tube navigation. We combined a lab-on-a-chip device with a microelectromechanical systems-based force sensor to mimic the pollen tube's journey from stigma to ovary in vitro. A force-sensing plate creates a mechanical obstacle for the pollen tube to either circumvent or attempt to penetrate while measuring the involved forces in real time. The change of growth behavior and intracellular signaling activities can be observed with a fluorescence microscope.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:580 Plants (Botany)
Scopus Subject Areas:Life Sciences > Molecular Biology
Life Sciences > Genetics
Language:English
Date:1 January 2020
Deposited On:22 Jan 2021 09:34
Last Modified:25 Jan 2021 16:09
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:2160
ISSN:1064-3745
ISBN:978-1-0716-0671-1
Additional Information:This is a post-peer-review, pre-copyedit version of an article published in Pollen and Pollen Tube Biology : Methods and Protocols. The final authenticated version is available online at: https://doi.org/10.1007/978-1-0716-0672-8_20
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-0716-0672-8_20
PubMed ID:32529444

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