Header

UZH-Logo

Maintenance Infos

Selection enhances protein evolvability by increasing mutational robustness and foldability


Zheng, Jia; Guo, Ning; Wagner, Andreas (2020). Selection enhances protein evolvability by increasing mutational robustness and foldability. Science, 370(6521):eabb5962.

Abstract

Natural selection can promote or hinder a population’s evolvability—the ability to evolve new and adaptive phenotypes—but the underlying mechanisms are poorly understood. To examine how the strength of selection affects evolvability, we subjected populations of yellow fluorescent protein to directed evolution under different selection regimes and then evolved them toward the new phenotype of green fluorescence. Populations under strong selection for the yellow phenotype evolved the green phenotype most rapidly. They did so by accumulating mutations that increase both robustness to mutations and foldability. Under weak selection, neofunctionalizing mutations rose to higher frequency at first, but more frequent deleterious mutations undermined their eventual success. Our experiments show how selection can enhance evolvability by enhancing robustness and create the conditions necessary for evolutionary success.

Abstract

Natural selection can promote or hinder a population’s evolvability—the ability to evolve new and adaptive phenotypes—but the underlying mechanisms are poorly understood. To examine how the strength of selection affects evolvability, we subjected populations of yellow fluorescent protein to directed evolution under different selection regimes and then evolved them toward the new phenotype of green fluorescence. Populations under strong selection for the yellow phenotype evolved the green phenotype most rapidly. They did so by accumulating mutations that increase both robustness to mutations and foldability. Under weak selection, neofunctionalizing mutations rose to higher frequency at first, but more frequent deleterious mutations undermined their eventual success. Our experiments show how selection can enhance evolvability by enhancing robustness and create the conditions necessary for evolutionary success.

Statistics

Citations

Altmetrics

Downloads

6 downloads since deposited on 17 Feb 2021
6 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
Dewey Decimal Classification:570 Life sciences; biology
590 Animals (Zoology)
Scopus Subject Areas:Health Sciences > Multidisciplinary
Uncontrolled Keywords:Multidisciplinary
Language:English
Date:4 December 2020
Deposited On:17 Feb 2021 08:53
Last Modified:18 Feb 2021 21:00
Publisher:American Association for the Advancement of Science
ISSN:0036-8075
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1126/science.abb5962
PubMed ID:33273072
Project Information:
  • : FunderSNSF
  • : Grant ID31003A_172887
  • : Project TitleRobustness and weakened selection in the adaptive evolution of fluorescent proteins

Download

Green Open Access

Download PDF  'Selection enhances protein evolvability by increasing mutational robustness and foldability'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 3MB
View at publisher