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Cortex‐wide microcirculation mapping with ultrafast large‐field multifocal illumination microscopy


Chen, Zhenyue; Zhou, Quanyu; Rebling, Johannes; Razansky, Daniel (2020). Cortex‐wide microcirculation mapping with ultrafast large‐field multifocal illumination microscopy. Journal of Biophotonics, 13(11):e202000198.

Abstract

The recently introduced large‐field multifocal illumination (LMI) fluorescence microscopy technique opened new possibilities for transcranial observations of mouse brain dynamics with a unique combination of capillary level resolution and centimeter‐scale field‐of‐view (FOV). Here we report on a new acceleration scheme for LMI based on raster scan of a lattice pattern combined with a parallel camera exposure scheme, which attains 200 Hz frame rate over 12 × 12 mm2 FOV with 7.5 μm spatial resolution. We demonstrate real‐time transcranial in vivo tracking of particles and imaging of microcirculation across the entire mouse cortex, thus corroborating the superb spatiotemporal resolution performance of LMI unattainable with other techniques. Potential applications include investigations into cerebrovascular function, cell tracking, as well as large‐scale functional neuroimaging.

Abstract

The recently introduced large‐field multifocal illumination (LMI) fluorescence microscopy technique opened new possibilities for transcranial observations of mouse brain dynamics with a unique combination of capillary level resolution and centimeter‐scale field‐of‐view (FOV). Here we report on a new acceleration scheme for LMI based on raster scan of a lattice pattern combined with a parallel camera exposure scheme, which attains 200 Hz frame rate over 12 × 12 mm2 FOV with 7.5 μm spatial resolution. We demonstrate real‐time transcranial in vivo tracking of particles and imaging of microcirculation across the entire mouse cortex, thus corroborating the superb spatiotemporal resolution performance of LMI unattainable with other techniques. Potential applications include investigations into cerebrovascular function, cell tracking, as well as large‐scale functional neuroimaging.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology

04 Faculty of Medicine > Institute of Biomedical Engineering
Dewey Decimal Classification:170 Ethics
610 Medicine & health
Scopus Subject Areas:Physical Sciences > General Chemistry
Physical Sciences > General Materials Science
Life Sciences > General Biochemistry, Genetics and Molecular Biology
Physical Sciences > General Engineering
Physical Sciences > General Physics and Astronomy
Uncontrolled Keywords:General Engineering, General Biochemistry, Genetics and Molecular Biology, General Physics and Astronomy, General Materials Science, General Chemistry
Language:English
Date:1 November 2020
Deposited On:01 Feb 2021 15:42
Last Modified:02 Feb 2021 21:00
Publisher:Wiley-VCH Verlag
ISSN:1864-063X
OA Status:Closed
Publisher DOI:https://doi.org/10.1002/jbio.202000198
PubMed ID:32761746
Project Information:
  • : FunderH2020
  • : Grant ID746430
  • : Project TitleMSIOAM - Multifocal structured illumination optoacoustic microscopy

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