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Widefield fluorescence localization microscopy for transcranial imaging of cortical perfusion with capillary resolution


Chen, Zhenyue; Zhou, Quanyu; Robin, Justine; Razansky, Daniel (2020). Widefield fluorescence localization microscopy for transcranial imaging of cortical perfusion with capillary resolution. Optics letters, 45(13):3470.

Abstract

Imaging of cerebral vasculature is impeded with the existing fluorescence microscopy methods due to intense light scattering in living tissues and the need for highly invasive craniotomy procedures to resolve structures on a capillary scale. We propose a widefield fluorescence localization microscopy technique for high-resolution transcranial imaging and quantitative assessment of cortical perfusion in mice. The method is based on tracking single fluorescent microparticles sparsely distributed in the blood stream using a simple CMOS camera and a continuous-wave laser source. We demonstrate quantitative transcranial in vivo mapping of the blood flow velocity and direction at capillary level resolution (5 µm) across the entire cortex. The new technique opens a new high-resolution transcranial window into the brain function in health and disease.

Abstract

Imaging of cerebral vasculature is impeded with the existing fluorescence microscopy methods due to intense light scattering in living tissues and the need for highly invasive craniotomy procedures to resolve structures on a capillary scale. We propose a widefield fluorescence localization microscopy technique for high-resolution transcranial imaging and quantitative assessment of cortical perfusion in mice. The method is based on tracking single fluorescent microparticles sparsely distributed in the blood stream using a simple CMOS camera and a continuous-wave laser source. We demonstrate quantitative transcranial in vivo mapping of the blood flow velocity and direction at capillary level resolution (5 µm) across the entire cortex. The new technique opens a new high-resolution transcranial window into the brain function in health and disease.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology

04 Faculty of Medicine > Institute of Biomedical Engineering
Dewey Decimal Classification:170 Ethics
610 Medicine & health
Scopus Subject Areas:Physical Sciences > Atomic and Molecular Physics, and Optics
Uncontrolled Keywords:Atomic and Molecular Physics, and Optics
Language:English
Date:1 July 2020
Deposited On:01 Feb 2021 15:54
Last Modified:02 Feb 2021 21:00
Publisher:Optical Society of America
ISSN:0146-9592
OA Status:Closed
Publisher DOI:https://doi.org/10.1364/ol.396123
PubMed ID:32630874

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