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Development and characterization of novel SSR markers in the endangered endemic species Ferula sadleriana


Malkócs, Tamás; Laczkó, Levente; Bereczki, Judit; Meglécz, Emese; Szövényi, Péter; Sramkó, Gábor (2020). Development and characterization of novel SSR markers in the endangered endemic species Ferula sadleriana. Applications in Plant Sciences, 8(2):e11321.

Abstract

Premise: Ferula sadleriana (Apiaceae) is a polycarpic, perennial herb with a very limited range and small populations. It is listed as "endangered" on the IUCN Red List of Threatened Species. Microsatellite markers can contribute to conservation efforts by allowing the study of the genetic structure of its shrinking populations.

Methods and results: We used a microsatellite-enriched library combined with an Illumina sequencing approach to develop simple sequence repeat markers in our target species. Out of 44 tested primer pairs, 22 provided specific products, and 13 showed heterologous amplification in the target species. Cross-species amplification was achieved at 20 and 19 loci in two congeneric species, F. soongarica and F. tatarica, respectively.

Conclusions: The primers described here are the first tools that enable the population genetic characterization of F. sadleriana. Our results suggest a wider applicability in the genus Ferula.

Abstract

Premise: Ferula sadleriana (Apiaceae) is a polycarpic, perennial herb with a very limited range and small populations. It is listed as "endangered" on the IUCN Red List of Threatened Species. Microsatellite markers can contribute to conservation efforts by allowing the study of the genetic structure of its shrinking populations.

Methods and results: We used a microsatellite-enriched library combined with an Illumina sequencing approach to develop simple sequence repeat markers in our target species. Out of 44 tested primer pairs, 22 provided specific products, and 13 showed heterologous amplification in the target species. Cross-species amplification was achieved at 20 and 19 loci in two congeneric species, F. soongarica and F. tatarica, respectively.

Conclusions: The primers described here are the first tools that enable the population genetic characterization of F. sadleriana. Our results suggest a wider applicability in the genus Ferula.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Systematic and Evolutionary Botany
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:580 Plants (Botany)
Scopus Subject Areas:Life Sciences > Ecology, Evolution, Behavior and Systematics
Life Sciences > Plant Science
Language:English
Date:1 February 2020
Deposited On:02 Feb 2021 07:12
Last Modified:06 Feb 2021 04:31
Publisher:Botanical Society of America
ISSN:2168-0450
OA Status:Gold
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1002/aps3.11321
PubMed ID:32110501
Project Information:
  • : FunderSNSF
  • : Grant ID31003A_160004
  • : Project TitleTesting hypotheses on the evolutionary origin of the land plant sporophyte
  • : FunderSNSF
  • : Grant IDPZ00P3_131726
  • : Project TitleGene expression and the evolution of plant alternation of generations

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