Header

UZH-Logo

Maintenance Infos

MsrR contributes to cell surface characteristics and virulence in Staphylococcus aureus


Hübscher, J; McCallum, N; Sifri, C D; Majcherczyk, P A; Entenza, J M; Heusser, R; Berger-Bächi, B; Stutzmann Meier, P (2009). MsrR contributes to cell surface characteristics and virulence in Staphylococcus aureus. FEMS Microbiology Letters, 295(2):251-260.

Abstract

MsrR, a factor contributing to methicillin resistance in Staphylococcus aureus, belongs to the LytR-CpsA-Psr family of cell envelope-associated proteins. Deletion of msrR increased cell size and aggregation, and altered envelope properties, leading to a temporary reduction in cell surface hydrophobicity, diminished colony-spreading ability, and an increased susceptibility to Congo red. The reduced phosphorus content of purified cell walls of the msrR mutant suggested a reduction in wall teichoic acids, which may explain some of the observed phenotypes. Microarray analysis of the msrR deletion mutant revealed only minor changes in the global transcriptome, suggesting that MsrR has structural rather than regulatory functions. Importantly, virulence of the msrR mutant was decreased in a nematode-killing assay as well as in rat experimental endocarditis. MsrR is therefore likely to play a role in cell envelope maintenance, cell separation, and pathogenicity of S. aureus.

Abstract

MsrR, a factor contributing to methicillin resistance in Staphylococcus aureus, belongs to the LytR-CpsA-Psr family of cell envelope-associated proteins. Deletion of msrR increased cell size and aggregation, and altered envelope properties, leading to a temporary reduction in cell surface hydrophobicity, diminished colony-spreading ability, and an increased susceptibility to Congo red. The reduced phosphorus content of purified cell walls of the msrR mutant suggested a reduction in wall teichoic acids, which may explain some of the observed phenotypes. Microarray analysis of the msrR deletion mutant revealed only minor changes in the global transcriptome, suggesting that MsrR has structural rather than regulatory functions. Importantly, virulence of the msrR mutant was decreased in a nematode-killing assay as well as in rat experimental endocarditis. MsrR is therefore likely to play a role in cell envelope maintenance, cell separation, and pathogenicity of S. aureus.

Statistics

Citations

Dimensions.ai Metrics
31 citations in Web of Science®
31 citations in Scopus®
27 citations in Microsoft Academic
Google Scholar™

Altmetrics

Downloads

1 download since deposited on 29 Jul 2009
1 download since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Microbiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2009
Deposited On:29 Jul 2009 07:26
Last Modified:29 Aug 2018 16:26
Publisher:Wiley-Blackwell
ISSN:0378-1097
Additional Information:The definitive version is available at www.blackwell-synergy.com
OA Status:Green
Publisher DOI:https://doi.org/10.1111/j.1574-6968.2009.01603.x
PubMed ID:19459977

Download

Download PDF  'MsrR contributes to cell surface characteristics and virulence in Staphylococcus aureus'.
Preview
Content: Published Version
Language: English
Filetype: PDF (Nationallizenz 142-005)
Size: 1MB
View at publisher