Navigation auf zora.uzh.ch

Search ZORA

ZORA (Zurich Open Repository and Archive)

Migration von ZORA auf die Software DSpace

ZORA will change to a new software on 8th September 2025. Please note: deadline for new submissions is 21th July 2025!

Information & dates for training courses can be found here: Information on Software Migration.

Different Behavior of the Histidine Residue toward Cadmium and Zinc in a Cadmium-Specific Metallothionein from an Aquatic Fungus

Perinelli, Monica; Tegoni, Matteo; Freisinger, Eva (2020). Different Behavior of the Histidine Residue toward Cadmium and Zinc in a Cadmium-Specific Metallothionein from an Aquatic Fungus. Inorganic Chemistry, 59(23):16988-16997.

Abstract

Metallothioneins (MTs) are a large superfamily of ubiquitous cysteine-rich metalloproteins with main functions in metal ion homeostasis and detoxification. Neclu_MT1 is a metallothionein from the aquatic fungus Heliscus lugdunensis and so far the only known MT that is solely induced by CdII but not by ZnII or copper ions. In addition to eight cysteine residues, Neclu_MT1 also contains a less common single C-terminal histidine residue. To better understand the role of this histidine residue in metal ion binding, for the first time, potentiometric pH titrations are applied, revealing insights into the protonation and metal ion binding processes. Additional studies with absorption and NMR spectroscopy complement the finding that while the histidine residue is not crucial for the overall metal binding capacity, it does serve as a ligand in the ZnII but not in the CdII form of the protein.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Scopus Subject Areas:Physical Sciences > Physical and Theoretical Chemistry
Physical Sciences > Inorganic Chemistry
Uncontrolled Keywords:Physical and Theoretical Chemistry, Inorganic Chemistry
Language:English
Date:7 December 2020
Deposited On:03 Feb 2021 16:02
Last Modified:23 Jun 2025 02:08
Publisher:American Chemical Society (ACS)
ISSN:0020-1669
OA Status:Hybrid
Publisher DOI:https://doi.org/10.1021/acs.inorgchem.0c02171
Project Information:
  • Funder: SNSF
  • Grant ID: 200020_175623
  • Project Title: Cluster diversity in plant and fungi metallothioneins - Properties and structures as a gate to functions
Download PDF  'Different Behavior of the Histidine Residue toward Cadmium and Zinc in a Cadmium-Specific Metallothionein from an Aquatic Fungus'.
Preview
  • Content: Accepted Version

Metadata Export

Statistics

Citations

Dimensions.ai Metrics
9 citations in Web of Science®
10 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

86 downloads since deposited on 03 Feb 2021
18 downloads since 12 months
Detailed statistics

Authors, Affiliations, Collaborations

Similar Publications