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Effects of endodontic irrigants on blood and blood-stained dentin


Zollinger, Adrian. Effects of endodontic irrigants on blood and blood-stained dentin. 2020, University of Zurich, Faculty of Medicine.

Abstract

Objectives: This study aimed to investigate bleaching effects of common endodontic irrigants on human whole blood and blood-stained dentin. Specifically, it was assessed whether sodium hypochlorite at a clinically recommended concentration (2.5% NaOCl) would bleach with similar efficacy as a peroxide-based irrigant at higher molarity (5% H2O2). Furthermore, the effects of a NaOCl-compatible chelator with a high affinity to iron (Dual Rinse HEDP) were investigated.

Methods: Human whole blood was mixed at a 1:20 ratio with either phosphate-buffered saline, 9% HEDP, 2.5% NaOCl, 2.5% NaOCl containing 9% HEDP, or 5% H2O2. Effects were assessed spectrometrically and photographically. Human dentin specimens were prepared with a methacrylate reservoir for liquids and a polished assessment side over 1 mm dentin thickness. Dentin was stained using human whole blood for 3 weeks and subsequently exposed to the irrigants for 60 min. Measurements were performed in the CIELAB color space. Results were compared using parametric tests with the alpha-type error set to 5%.

Results: When directly exposed, the solutions containing NaOCl completely discolored the blood, while the 5% H2O2 exerted a bleaching effect without complete dissolution of dissolved matter, and the pure 9% HEDP had no effect at all. The NaOCl solutions bleached blood-stained dentin more efficiently than H2O2 (p < 0.05).

Conclusions: Under the current conditions, the 2.5% NaOCl solution had a stronger bleaching effect on blood and blood-stained dentin than 5% H2O2. HEDP did not have any direct impact on blood color or NaOCl-derived bleaching.

Keywords: Dentistry.

Abstract

Objectives: This study aimed to investigate bleaching effects of common endodontic irrigants on human whole blood and blood-stained dentin. Specifically, it was assessed whether sodium hypochlorite at a clinically recommended concentration (2.5% NaOCl) would bleach with similar efficacy as a peroxide-based irrigant at higher molarity (5% H2O2). Furthermore, the effects of a NaOCl-compatible chelator with a high affinity to iron (Dual Rinse HEDP) were investigated.

Methods: Human whole blood was mixed at a 1:20 ratio with either phosphate-buffered saline, 9% HEDP, 2.5% NaOCl, 2.5% NaOCl containing 9% HEDP, or 5% H2O2. Effects were assessed spectrometrically and photographically. Human dentin specimens were prepared with a methacrylate reservoir for liquids and a polished assessment side over 1 mm dentin thickness. Dentin was stained using human whole blood for 3 weeks and subsequently exposed to the irrigants for 60 min. Measurements were performed in the CIELAB color space. Results were compared using parametric tests with the alpha-type error set to 5%.

Results: When directly exposed, the solutions containing NaOCl completely discolored the blood, while the 5% H2O2 exerted a bleaching effect without complete dissolution of dissolved matter, and the pure 9% HEDP had no effect at all. The NaOCl solutions bleached blood-stained dentin more efficiently than H2O2 (p < 0.05).

Conclusions: Under the current conditions, the 2.5% NaOCl solution had a stronger bleaching effect on blood and blood-stained dentin than 5% H2O2. HEDP did not have any direct impact on blood color or NaOCl-derived bleaching.

Keywords: Dentistry.

Statistics

Additional indexing

Item Type:Dissertation (monographical)
Referees:Attin Thomas, Mohn Dirk, Zehnder Matthias
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic of Conservative and Preventive Dentistry
UZH Dissertations
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2020
Deposited On:25 Feb 2021 12:53
Last Modified:21 Feb 2023 09:41
Additional Information:Diese Dissertation wurde im Journal Heliyon mit folgender Zitation Zollinger, Adrian, Effects of endodontic irrigants on blood and blood-stained dentin, 2020, University of Zurich, Medizinische Fakultät und doi.org/10.1016/j.heliyon.2019.e01794 publiziert.
OA Status:Closed
Free access at:Related URL. An embargo period may apply.
Related URLs:https://www.zora.uzh.ch/id/eprint/183115/
https://pubmed.ncbi.nlm.nih.gov/31193548/
https://www.cell.com/heliyon/fulltext/S2405-8440(18)38499-8?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2405844018384998%3Fshowall%3Dtrue
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