Navigation auf zora.uzh.ch

Search ZORA

ZORA (Zurich Open Repository and Archive)

Prevalence and molecular characterization of C. pecorum detected in Swiss fattening pigs

Rohner, Lea; Marti, Hanna; Torgerson, Paul; Hoffmann, Karolin; Jelocnik, Martina; Borel, Nicole (2021). Prevalence and molecular characterization of C. pecorum detected in Swiss fattening pigs. Veterinary Microbiology, 256:109062.

Abstract

Chlamydia (C.) pecorum, an obligate intracellular bacterial species commonly found in ruminants, can also occur in pigs. However, its significance as a potential porcine pathogen, or commensal, is still unclear. In a previous study (Hoffmann et al. 2015), mixed infections of C. suis and C. pecorum were detected in 14 Swiss fattening pig farms. Using these samples, we aimed to investigate the infection dynamics of C. suis and C. pecorum mixed infections in these farms. In addition, we analyzed the genetic diversity of Swiss porcine C. pecorum strains in relation to globally circulating strains. In total, 1284 conjunctival and rectal swabs from 391 pigs, collected at the beginning and end of the fattening period, were tested during the course of this study. We determined the bacterial loads of C. suis and C. pecorum using species-specific real-time PCR (qPCR) and compared these results to already existing DNA-microarray and Chlamydiaceae qPCR data. Overall, C. suis and Chlamydiaceae copy numbers decreased in the course of the fattening period, whereas C. pecorum copy numbers increased. No association was found between clinical signs (conjunctivitis, lameness and diarrhea) and the bacterial loads. Preventive antibiotic treatment at the beginning of the fattening period significantly lowered the chlamydial load and outdoor access was associated with higher loads. Proximity to the nearest ruminants correlated with increased C. pecorum loads, indicating that C. pecorum could be transmitted from ruminants to pigs. Multi-locus sequence typing (MLST) and major outer membrane protein (ompA) genotyping revealed two novel sequence types (STs) (301, 302) and seven unique ompA genotypes (1-7) that appear to form a specific clade separate from other European C. pecorum strains

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinärwissenschaftliches Institut > Institute of Veterinary Pathology
05 Vetsuisse Faculty > Veterinärwissenschaftliches Institut > Chair in Veterinary Epidemiology
05 Vetsuisse Faculty > Center for Clinical Studies
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Microbiology
Health Sciences > General Veterinary
Uncontrolled Keywords:Microbiology, General Veterinary, General Medicine
Language:English
Date:1 March 2021
Deposited On:07 May 2021 08:56
Last Modified:25 Dec 2024 02:37
Publisher:Elsevier
ISSN:0378-1135
OA Status:Hybrid
Publisher DOI:https://doi.org/10.1016/j.vetmic.2021.109062
Download PDF  'Prevalence and molecular characterization of C. pecorum detected in Swiss fattening pigs'.
Preview
  • Content: Published Version
  • Language: English
  • Licence: Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Download PDF  'Prevalence and molecular characterization of C. pecorum detected in Swiss fattening pigs'.
Preview
  • Content: Accepted Version
  • Language: English
  • Licence: Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Metadata Export

Statistics

Citations

Dimensions.ai Metrics
7 citations in Web of Science®
7 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

80 downloads since deposited on 07 May 2021
35 downloads since 12 months
Detailed statistics

Authors, Affiliations, Collaborations

Similar Publications