Header

UZH-Logo

Maintenance Infos

3D mechanical characterization of single cells and small organisms using acoustic manipulation and force microscopy


Läubli, Nino F; Burri, Jan T; Marquard, Julian; Vogler, Hannes; Mosca, Gabriella; Vertti-Quintero, Nadia; Shamsudhin, Naveen; deMello, Andrew; Grossniklaus, Ueli; Ahmed, Daniel; Nelson, Bradley J (2021). 3D mechanical characterization of single cells and small organisms using acoustic manipulation and force microscopy. Nature Communications, 12:2583.

Abstract

Quantitative micromechanical characterization of single cells and multicellular tissues or organisms is of fundamental importance to the study of cellular growth, morphogenesis, and cell-cell interactions. However, due to limited manipulation capabilities at the microscale, systems used for mechanical characterizations struggle to provide complete three-dimensional coverage of individual specimens. Here, we combine an acoustically driven manipulation device with a micro-force sensor to freely rotate biological samples and quantify mechanical properties at multiple regions of interest within a specimen. The versatility of this tool is demonstrated through the analysis of single Lilium longiflorum pollen grains, in combination with numerical simulations, and individual Caenorhabditis elegans nematodes. It reveals local variations in apparent stiffness for single specimens, providing previously inaccessible information and datasets on mechanical properties that serve as the basis for biophysical modelling and allow deeper insights into the biomechanics of these living systems.

Abstract

Quantitative micromechanical characterization of single cells and multicellular tissues or organisms is of fundamental importance to the study of cellular growth, morphogenesis, and cell-cell interactions. However, due to limited manipulation capabilities at the microscale, systems used for mechanical characterizations struggle to provide complete three-dimensional coverage of individual specimens. Here, we combine an acoustically driven manipulation device with a micro-force sensor to freely rotate biological samples and quantify mechanical properties at multiple regions of interest within a specimen. The versatility of this tool is demonstrated through the analysis of single Lilium longiflorum pollen grains, in combination with numerical simulations, and individual Caenorhabditis elegans nematodes. It reveals local variations in apparent stiffness for single specimens, providing previously inaccessible information and datasets on mechanical properties that serve as the basis for biophysical modelling and allow deeper insights into the biomechanics of these living systems.

Statistics

Citations

Dimensions.ai Metrics
48 citations in Web of Science®
44 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

31 downloads since deposited on 19 May 2021
4 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:580 Plants (Botany)
Scopus Subject Areas:Physical Sciences > General Chemistry
Life Sciences > General Biochemistry, Genetics and Molecular Biology
Physical Sciences > General Physics and Astronomy
Uncontrolled Keywords:General Biochemistry, Genetics and Molecular Biology, General Physics and Astronomy, General Chemistry
Language:English
Date:2021
Deposited On:19 May 2021 08:56
Last Modified:26 May 2024 01:39
Publisher:Nature Publishing Group
ISSN:2041-1723
OA Status:Gold
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1038/s41467-021-22718-8
PubMed ID:33972516
Project Information:
  • : FunderSNSF
  • : Grant IDCR22I2_166110
  • : Project TitleMechanical Basis for the Convergent Evolution of Sensory Hairs in Animals and Plants
  • Content: Published Version
  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)