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Identification of Active Transposable Elements in Plants: The Mobilome-Seq Approach


Thieme, Michael; Roulin, Anne C (2021). Identification of Active Transposable Elements in Plants: The Mobilome-Seq Approach. In: Cho, Jungnam. Plant Transposable Elements. New York: Springer, 95-102.

Abstract

Transposable elements (TEs) are the main component of eukaryotic genomes. Besides their impact on genome size, TEs are also functionally important as they can alter gene expression and influence phenotypic variation. In plants, most top-down studies focus on extremely clear phenotypes such as the shape or the color of individuals and do not explore fully the role of TEs in evolution. Assessing the impact of TEs in a more systematic manner, however, requires identifying active TEs to further study their impact on phenotypes. In this chapter, we describe an in planta approach that consists in activating TEs by interfering with pathways involved in their silencing. It enables to directly investigate the functional impact of single TE families at low cost.

Abstract

Transposable elements (TEs) are the main component of eukaryotic genomes. Besides their impact on genome size, TEs are also functionally important as they can alter gene expression and influence phenotypic variation. In plants, most top-down studies focus on extremely clear phenotypes such as the shape or the color of individuals and do not explore fully the role of TEs in evolution. Assessing the impact of TEs in a more systematic manner, however, requires identifying active TEs to further study their impact on phenotypes. In this chapter, we describe an in planta approach that consists in activating TEs by interfering with pathways involved in their silencing. It enables to directly investigate the functional impact of single TE families at low cost.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Zurich-Basel Plant Science Center
08 Research Priority Programs > Evolution in Action: From Genomes to Ecosystems
Dewey Decimal Classification:580 Plants (Botany)
Scopus Subject Areas:Life Sciences > Molecular Biology
Life Sciences > Genetics
Language:English
Date:1 January 2021
Deposited On:19 May 2021 14:48
Last Modified:24 Feb 2024 02:50
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:2250
ISSN:1064-3745
ISBN:978-1-0716-1133-3
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-0716-1134-0_8
PubMed ID:33900595