Header

UZH-Logo

Maintenance Infos

Recommendations for the introduction of metagenomic next-generation sequencing in clinical virology, part II: bioinformatic analysis and reporting


de Vries, Jutte J C; Brown, Julianne R; Couto, Natacha; Beer, Martin; Le Mercier, Philippe; Sidorov, Igor; Papa, Anna; Fischer, Nicole; Oude Munnink, Bas B; Rodriquez, Christophe; Zaheri, Maryam; Sayiner, Arzu; Hönemann, Mario; Pérez-Cataluña, Alba; Carbo, Ellen C; Bachofen, Claudia; Kubacki, Jakub; et al (2021). Recommendations for the introduction of metagenomic next-generation sequencing in clinical virology, part II: bioinformatic analysis and reporting. Journal of Clinical Virology, 138:104812.

Abstract

Metagenomic next-generation sequencing (mNGS) is an untargeted technique for determination of microbial DNA/RNA sequences in a variety of sample types from patients with infectious syndromes. mNGS is still in its early stages of broader translation into clinical applications. To further support the development, implementation, optimization and standardization of mNGS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mNGS for viral diagnostics to share methodologies and experiences, and to develop application guidelines. Following the ENNGS publication Recommendations for the introduction of mNGS in clinical virology, part I: wet lab procedure in this journal, the current manuscript aims to provide practical recommendations for the bioinformatic analysis of mNGS data and reporting of results to clinicians.

Abstract

Metagenomic next-generation sequencing (mNGS) is an untargeted technique for determination of microbial DNA/RNA sequences in a variety of sample types from patients with infectious syndromes. mNGS is still in its early stages of broader translation into clinical applications. To further support the development, implementation, optimization and standardization of mNGS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mNGS for viral diagnostics to share methodologies and experiences, and to develop application guidelines. Following the ENNGS publication Recommendations for the introduction of mNGS in clinical virology, part I: wet lab procedure in this journal, the current manuscript aims to provide practical recommendations for the bioinformatic analysis of mNGS data and reporting of results to clinicians.

Statistics

Citations

Dimensions.ai Metrics
37 citations in Web of Science®
36 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

136 downloads since deposited on 18 Jun 2021
34 downloads since 12 months
Detailed statistics

Additional indexing

Contributors:the ESCV Network on Next-Generation Sequencing
Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Virology
05 Vetsuisse Faculty > Veterinärwissenschaftliches Institut > Institute of Virology
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Virology
Health Sciences > Infectious Diseases
Uncontrolled Keywords:Virology, Infectious Diseases
Language:English
Date:1 May 2021
Deposited On:18 Jun 2021 07:45
Last Modified:25 Jun 2024 01:40
Publisher:Elsevier
ISSN:1386-6532
OA Status:Hybrid
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/j.jcv.2021.104812
PubMed ID:33819811
  • Content: Published Version
  • Language: English
  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)