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Establishing standardized immune phenotyping of metastatic melanoma by digital pathology

Sobottka-Brillout, Bettina; Nowak, Marta; Frei, Anja Laura; Haberecker, Martina; Merki, Samuel; Dummer, Reinhard; Levesque, Mitchell P; Moch, Holger; Koelzer, Viktor Hendrik (2021). Establishing standardized immune phenotyping of metastatic melanoma by digital pathology. Laboratory investigation, 101(12):1561-1570.

Abstract

CD8+ tumor-infiltrating T cells can be regarded as one of the most relevant predictive biomarkers in immune-oncology. Highly infiltrated tumors, referred to as inflamed (clinically "hot"), show the most favorable response to immune checkpoint inhibitors in contrast to tumors with a scarce immune infiltrate called immune desert or excluded (clinically "cold"). Nevertheless, quantitative and reproducible methods examining their prevalence within tumors are lacking. We therefore established a computational diagnostic algorithm to quantitatively measure spatial densities of tumor-infiltrating CD8+ T cells by digital pathology within the three known tumor compartments as recommended by the International Immuno-Oncology Biomarker Working Group in 116 prospective metastatic melanomas of the Swiss Tumor Profiler cohort. Workflow robustness was confirmed in 33 samples of an independent retrospective validation cohort. The introduction of the intratumoral tumor center compartment proved to be most relevant for establishing an immune diagnosis in metastatic disease, independent of metastatic site. Cut-off values for reproducible classification were defined and successfully assigned densities into the respective immune diagnostic category in the validation cohort with high sensitivity, specificity, and precision. We provide a robust diagnostic algorithm based on intratumoral and stromal CD8+ T-cell densities in the tumor center compartment that translates spatial densities of tumor-infiltrating CD8+ T cells into the clinically relevant immune diagnostic categories "inflamed", "excluded", and "desert". The consideration of the intratumoral tumor center compartment allows immune phenotyping in the clinically highly relevant setting of metastatic lesions, even if the invasive margin compartment is not captured in biopsy material.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Oncology and Hematology
04 Faculty of Medicine > University Hospital Zurich > Institute of Pathology and Molecular Pathology
04 Faculty of Medicine > Epidemiology, Biostatistics and Prevention Institute (EBPI)
04 Faculty of Medicine > University Hospital Zurich > Dermatology Clinic
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Health Sciences > Pathology and Forensic Medicine
Life Sciences > Molecular Biology
Life Sciences > Cell Biology
Language:English
Date:1 December 2021
Deposited On:09 Sep 2021 11:16
Last Modified:26 Oct 2024 01:35
Publisher:Nature Publishing Group
ISSN:0023-6837
OA Status:Hybrid
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1038/s41374-021-00653-y
Related URLs:https://www.zora.uzh.ch/id/eprint/207719/
PubMed ID:34446805
Other Identification Number:Erratum zu diesem Artikel: 10.1038/s41374-021-00676-5 (DOI)
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  • Language: English
  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)

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