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Measuring Circadian Rhythms in Human Cells


Du, Ngoc-Hien; Brown, Steven A (2021). Measuring Circadian Rhythms in Human Cells. In: Brown, Steven A. Circadian Clocks : Methods and Protocols. New York: Springer, 53-67.

Abstract

Human cells, especially primary fibroblasts from skin punch biopsy, have emerged over the last decade as powerful, unlimited, and easily accessible resources that bridge the gap between animal models and human subjects in basic as well as clinical research. The cells also retain molecular circadian clocks that reflect subject-specific differences in circadian physiology, and the cellular rhythms can be measured easily in large scale. This is a series of protocols that describes the procedure to measure circadian rhythms in these cells, starting from deriving fibroblasts from skin punch biopsy, to generation of stable cells expressing a circadian reporter, and finally measurement of cellular rhythms in large scale.

Abstract

Human cells, especially primary fibroblasts from skin punch biopsy, have emerged over the last decade as powerful, unlimited, and easily accessible resources that bridge the gap between animal models and human subjects in basic as well as clinical research. The cells also retain molecular circadian clocks that reflect subject-specific differences in circadian physiology, and the cellular rhythms can be measured easily in large scale. This is a series of protocols that describes the procedure to measure circadian rhythms in these cells, starting from deriving fibroblasts from skin punch biopsy, to generation of stable cells expressing a circadian reporter, and finally measurement of cellular rhythms in large scale.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > Molecular Biology
Life Sciences > Genetics
Language:English
Date:1 January 2021
Deposited On:13 Sep 2021 08:39
Last Modified:25 Jun 2024 01:43
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:2130
ISSN:1064-3745
ISBN:978-1-0716-0380-2
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-0716-0381-9_4
Official URL:https://link.springer.com/protocol/10.1007%2F978-1-0716-0381-9_4