Header

UZH-Logo

Maintenance Infos

A protocol for simultaneous Ca2+ and morphology imaging of brain endothelial tip cells in larval zebrafish


Liu, Ting-ting; Hou, Han; Du, Jiu-lin (2021). A protocol for simultaneous Ca2+ and morphology imaging of brain endothelial tip cells in larval zebrafish. STAR Protocols, 2(1):100388.

Abstract

Endothelial tip cells (ETCs) located at growing blood vessels display high morphological dynamics and associated intracellular Ca2+ activities with different spatiotemporal patterns during migration. Examining the Ca2+ activity and morphological dynamics of ETCs will provide an insight for understanding the mechanism of vascular development in organs, including the brain. Here, we describe a method for simultaneous monitoring and relevant analysis of the Ca2+ activity and morphology of growing brain ETCs in larval zebrafish.

Abstract

Endothelial tip cells (ETCs) located at growing blood vessels display high morphological dynamics and associated intracellular Ca2+ activities with different spatiotemporal patterns during migration. Examining the Ca2+ activity and morphological dynamics of ETCs will provide an insight for understanding the mechanism of vascular development in organs, including the brain. Here, we describe a method for simultaneous monitoring and relevant analysis of the Ca2+ activity and morphology of growing brain ETCs in larval zebrafish.

Statistics

Citations

Altmetrics

Downloads

17 downloads since deposited on 02 Nov 2021
5 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, further contribution
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > General Biochemistry, Genetics and Molecular Biology
Life Sciences > General Neuroscience
Life Sciences > General Immunology and Microbiology
Language:English
Date:1 March 2021
Deposited On:02 Nov 2021 10:36
Last Modified:25 Feb 2024 02:47
Publisher:Cell Press (Elsevier)
ISSN:2666-1667
OA Status:Gold
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/j.xpro.2021.100388
PubMed ID:33778782
Project Information:
  • : FunderChinese Academy of Sciences
  • : Grant ID
  • : Project Title
  • : FunderShanghai Municipality Science and Technology Commission
  • : Grant ID
  • : Project Title
  • Content: Published Version
  • Licence: Creative Commons: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)