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The involvement of hypoxia-inducible factor 1α (HIF1α)-stabilising factors in steroidogenic acute regulatory (STAR) protein-dependent steroidogenesis in murine KK1 granulosa cells in vitro

Gysin, Tina; Kowalewski, Mariusz P (2021). The involvement of hypoxia-inducible factor 1α (HIF1α)-stabilising factors in steroidogenic acute regulatory (STAR) protein-dependent steroidogenesis in murine KK1 granulosa cells in vitro. Reproduction, Fertility and Development, 33(18):865-880.

Abstract

As a component of hypoxia-inducible factor1 (HIF1)-complexes, HIF1α regulates the expression of steroidogenic acute regulatory (STAR) protein in granulosa cells. However, severe hypoxia or exaggeratedly expressed HIF1α have detrimental effects. HIF1α is regulated by factor inhibiting HIF (FIH), prolyl hydroxylases (PHD1, 2, 3) and von Hippel-Lindau (VHL) suppressor protein. In this study, the expression of FIH, PHD1, 2, 3 and VHL was investigated in murine ovaries and immortalised KK1 granulosa cells. We found FIH, VHL and PHD2 transcripts predominantly in growing tertiary follicles. Functional aspects were assessed in KK1 cells exposed to decreasing O2 (20%, 10%, 1%), by determining HIF1α, FIH, VHL, PHD1–3 and STAR expression. The main findings indicated gradually increasing PHD2 under lowered O2. Functional blocking of PHDs revealed biphasic effects on STAR expression; concomitantly with increasing HIF1α, STAR expression, which was initially induced, decreased significantly when HIF1α was strongly stabilised. Finally, PHD2 in particular might act as a specific regulator of HIF1α and, thereby, of STAR availability in granulosa cells.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinärwissenschaftliches Institut > Institute of Veterinary Anatomy
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Biotechnology
Health Sciences > Reproductive Medicine
Life Sciences > Animal Science and Zoology
Life Sciences > Molecular Biology
Life Sciences > Genetics
Life Sciences > Endocrinology
Life Sciences > Developmental Biology
Uncontrolled Keywords:Developmental Biology, Endocrinology, Genetics, Molecular Biology, Animal Science and Zoology, Reproductive Medicine, Biotechnology
Language:English
Date:1 January 2021
Deposited On:10 Jan 2022 15:45
Last Modified:26 Oct 2024 01:41
Publisher:C S I R O Publishing
ISSN:1031-3613
OA Status:Closed
Publisher DOI:https://doi.org/10.1071/rd21170
PubMed ID:34871543
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