Header

UZH-Logo

Maintenance Infos

Biohybrid microswimmers against bacterial infections


Shchelik, Inga S; Molino, João V D; Gademann, Karl (2021). Biohybrid microswimmers against bacterial infections. Acta Biomaterialia, 136:99-110.

Abstract

Biohybrid microswimmers exploit the natural abilities of motile microorganisms e.g. in releasing cargo on-demand. However, using such engineered swarms to release antibiotics addressing bacterial infections has not yet been realized. Herein, a design strategy for biohybrid microswimmers is reported, which fea- tures the covalent attachment of antibiotics with a photo-cleavable linker to the algae C hlamydomonas reinhardtii via two synthetic steps. This surface engineering does not rely on genetic manipulations, pro- ceeds with high efficiency, and retains the viability or phototaxis of microalgae. Two different antibi- otics have been separately utilized, which result in activity against both gram-positive and gram-negative strains. Guiding the biohybrid microswimmers by an external beacon, and on-demand delivery of the drugs by light with high spatial and temporal control, allowed for strong inhibition of bacterial growth. This efficient strategy could potentially allow for the selective treatment of bacterial infections by engi- neered algal microrobots with high precision in space and time.

Abstract

Biohybrid microswimmers exploit the natural abilities of motile microorganisms e.g. in releasing cargo on-demand. However, using such engineered swarms to release antibiotics addressing bacterial infections has not yet been realized. Herein, a design strategy for biohybrid microswimmers is reported, which fea- tures the covalent attachment of antibiotics with a photo-cleavable linker to the algae C hlamydomonas reinhardtii via two synthetic steps. This surface engineering does not rely on genetic manipulations, pro- ceeds with high efficiency, and retains the viability or phototaxis of microalgae. Two different antibi- otics have been separately utilized, which result in activity against both gram-positive and gram-negative strains. Guiding the biohybrid microswimmers by an external beacon, and on-demand delivery of the drugs by light with high spatial and temporal control, allowed for strong inhibition of bacterial growth. This efficient strategy could potentially allow for the selective treatment of bacterial infections by engi- neered algal microrobots with high precision in space and time.

Statistics

Citations

Dimensions.ai Metrics
5 citations in Web of Science®
12 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

86 downloads since deposited on 17 Jan 2022
63 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Scopus Subject Areas:Life Sciences > Biotechnology
Physical Sciences > Biomaterials
Life Sciences > Biochemistry
Physical Sciences > Biomedical Engineering
Life Sciences > Molecular Biology
Uncontrolled Keywords:Molecular Biology, Biomedical Engineering, Biochemistry, Biomaterials, General Medicine, Biotechnology
Language:English
Date:1 December 2021
Deposited On:17 Jan 2022 16:15
Last Modified:27 May 2024 01:49
Publisher:Elsevier
ISSN:1742-7061
OA Status:Hybrid
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/j.actbio.2021.09.048
Project Information:
  • : FunderSNSF
  • : Grant ID200021_182043
  • : Project TitleMechanism-Based Design, Synthesis, Biological Evaluation, and Delivery of Next-Generation Antibiotics
  • : FunderGebert Rüf Stiftung
  • : Grant ID
  • : Project Title
  • : FunderSchweizerische Eidgenossenschaft
  • : Grant ID
  • : Project Title
  • Content: Published Version
  • Language: English
  • Licence: Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)