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Exosomes as Carriers for Notch Molecules


Marconi, Guya Diletta; Diomede, Francesca; Trubiani, Oriana; Porcheri, Cristina; Mitsiadis, Thimios A (2022). Exosomes as Carriers for Notch Molecules. In: Dongyu, Jia. Notch Signaling Research : Methods and Protocols. New York, NY: Springer, 197-208.

Abstract

Exosomes are extracellular vesicles involved in cell-to-cell communication as well as extrusion of biological material. Using dental pulp stem cells culture as a model, we hereby describe a method for the packaging of Delta-like 4 (DLL4), a representative Notch ligand, into newly generated exosomes. We then provide methods of analysis to confirm the presence of Notch proteins and transcripts internalization and transport via exosomes.

Abstract

Exosomes are extracellular vesicles involved in cell-to-cell communication as well as extrusion of biological material. Using dental pulp stem cells culture as a model, we hereby describe a method for the packaging of Delta-like 4 (DLL4), a representative Notch ligand, into newly generated exosomes. We then provide methods of analysis to confirm the presence of Notch proteins and transcripts internalization and transport via exosomes.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Institute of Oral Biology
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Life Sciences > Molecular Biology
Life Sciences > Genetics
Language:English
Date:2022
Deposited On:29 Jun 2022 11:24
Last Modified:23 Mar 2024 04:41
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:2472
ISSN:1064-3745
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-0716-2201-8_16
PubMed ID:35674902
Other Identification Number:eBook ISBN978-1-0716-2201-8