Abstract
Artificial metalloenzymes (ArMs) are created by embedding a synthetic metal catalyst into a protein scaffold. ArMs have the potential to merge the catalytic advantages of natural enzymes with the reaction scope of synthetic catalysts. The choice of the protein scaffold is of utmost importance to tune the activity of the ArM. Herein, we show the repurposing of HaloTag, a self-labeling protein widely used in chemical biology, to create an ArM scaffold for metathesis. This monomeric protein scaffold allows for covalent attachment of metathesis cofactors, and the resulting ArMs are capable of catalyzing ring-closing metathesis. Both chemical and genetic engineering were explored to determine the evolvability of the resulting ArM. Additionally, exploration of the substrate scope revealed a reaction with promising turnover numbers (>48) and conversion rates (>96%).
Item Type: | Journal Article, not_refereed, original work |
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Communities & Collections: | 07 Faculty of Science > Department of Chemistry |
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Dewey Decimal Classification: | 540 Chemistry |
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Scopus Subject Areas: | Physical Sciences > Catalysis
Physical Sciences > General Chemistry |
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Uncontrolled Keywords: | Catalysis, General Chemistry |
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Language: | English |
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Date: | 21 May 2021 |
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Deposited On: | 09 Jan 2023 08:03 |
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Last Modified: | 26 Jun 2025 01:56 |
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Publisher: | American Chemical Society (ACS) |
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ISSN: | 2155-5435 |
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OA Status: | Green |
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Free access at: | PubMed ID. An embargo period may apply. |
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Publisher DOI: | https://doi.org/10.1021/acscatal.1c01470 |
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PubMed ID: | 34055452 |
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Project Information: | - Funder: SNSF
- Grant ID: 182046
- Project Title: Directed Evolution of Artificial Metalloproteins and Metalloenzymes
- Funder: H2020
- Grant ID: 694424
- Project Title: Directed Evolution of Artificial Metalloenzymes for In Vivo Applications
- Funder: Marie Skłodowska-Curie
- Grant ID: H2020-MSCA-IF-2017
- Project Title:
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