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Antibody Recognition of Cancer Cells via Glycan Surface Engineering


Szponarski, Mathieu; Gademann, Karl (2022). Antibody Recognition of Cancer Cells via Glycan Surface Engineering. Chembiochem, 23(15):e202200125.

Abstract

Stimulation of the body's immune system toward tumor cells is now well recognized as a promising strategy in cancer therapy. Just behind cell therapy and monoclonal antibodies, small molecule-based strategies are receiving growing attention as alternatives to direct immune response against tumor cells. However, the development of small-molecule approaches to modulate the balance between stimulatory immune factors and suppressive factors in a targeted way remains a challenge. Here, we report the cell surface functionalization of LS174T cancer cells with an abiotic hapten to recruit antibodies to the cell surface. Metabolic glycoengineering followed by covalent reaction with the hapten results in antibody recognition of the target cells. Microscopy and flow cytometry studies provide compelling evidence that metabolic glycoengineering and small molecule stimulators can be combined to direct antibody recognition.

Keywords: antibody; bioorganic chemistry; cancer; chemical biology; glycoengineering

Abstract

Stimulation of the body's immune system toward tumor cells is now well recognized as a promising strategy in cancer therapy. Just behind cell therapy and monoclonal antibodies, small molecule-based strategies are receiving growing attention as alternatives to direct immune response against tumor cells. However, the development of small-molecule approaches to modulate the balance between stimulatory immune factors and suppressive factors in a targeted way remains a challenge. Here, we report the cell surface functionalization of LS174T cancer cells with an abiotic hapten to recruit antibodies to the cell surface. Metabolic glycoengineering followed by covalent reaction with the hapten results in antibody recognition of the target cells. Microscopy and flow cytometry studies provide compelling evidence that metabolic glycoengineering and small molecule stimulators can be combined to direct antibody recognition.

Keywords: antibody; bioorganic chemistry; cancer; chemical biology; glycoengineering

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Scopus Subject Areas:Life Sciences > Biochemistry
Life Sciences > Molecular Medicine
Life Sciences > Molecular Biology
Physical Sciences > Organic Chemistry
Uncontrolled Keywords:Organic Chemistry, Molecular Biology, Molecular Medicine, Biochemistry
Language:English
Date:3 August 2022
Deposited On:09 Jan 2023 15:02
Last Modified:29 Mar 2024 02:37
Publisher:Wiley-VCH Verlag
ISSN:1439-4227
OA Status:Green
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1002/cbic.202200125
Related URLs:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400979/ (Library Catalogue)
PubMed ID:35638149
  • Content: Published Version
  • Language: English
  • Licence: Creative Commons: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)