Header

UZH-Logo

Maintenance Infos

Chromosome studies on bone marrow from Chinese hamsters treated with benzodiazepine tranquillizers and cyclophosphamide


Schmid, Werner; Staiger, G R (1969). Chromosome studies on bone marrow from Chinese hamsters treated with benzodiazepine tranquillizers and cyclophosphamide. Mutation research, 7(1):99-108.

Abstract

Chinese hamsters were treated for two weeks with high doses of chlordiazepoxide hydrochloride, diazepam, and medazepam. Chromosome preparations of bone marrow were made 5–8 h after the last drug application. Single chromatid breaks were found in 0.27% of the cells, an incidence not differing from control values. Cyclophosphamide was used as a known chromosome-breaking control substance in order to test the sensitivity and peculiarities of the bone marrow system. Doses of less than 16 mg/kg produced little chromosome damage whereas after doses of 32 mg/kg the incidence of chromosome aberrations was easily quantitated. The time interval between the last drug application and preparing the bone marrow proved to be of importance: after 10 applications of 64 mg/kg cyclophosphamide in a 14-day course of treatment, the percentage of metaphases with damaged chromosomes dropped from 96% at 6 h after the last medication to 16% at 24 h, 2–5% at 3 days, and to virtually zero after 6 days.

Abstract

Chinese hamsters were treated for two weeks with high doses of chlordiazepoxide hydrochloride, diazepam, and medazepam. Chromosome preparations of bone marrow were made 5–8 h after the last drug application. Single chromatid breaks were found in 0.27% of the cells, an incidence not differing from control values. Cyclophosphamide was used as a known chromosome-breaking control substance in order to test the sensitivity and peculiarities of the bone marrow system. Doses of less than 16 mg/kg produced little chromosome damage whereas after doses of 32 mg/kg the incidence of chromosome aberrations was easily quantitated. The time interval between the last drug application and preparing the bone marrow proved to be of importance: after 10 applications of 64 mg/kg cyclophosphamide in a 14-day course of treatment, the percentage of metaphases with damaged chromosomes dropped from 96% at 6 h after the last medication to 16% at 24 h, 2–5% at 3 days, and to virtually zero after 6 days.

Statistics

Citations

Dimensions.ai Metrics
98 citations in Web of Science®
83 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

0 downloads since deposited on 27 Jan 2023
0 downloads since 12 months

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Children's Hospital Zurich > Medical Clinic
04 Faculty of Medicine > Institute of Medical Genetics
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > Molecular Biology
Life Sciences > Genetics
Physical Sciences > Health, Toxicology and Mutagenesis
Uncontrolled Keywords:Health, Toxicology and Mutagenesis, Genetics, Molecular Biology
Language:English
Date:1 January 1969
Deposited On:27 Jan 2023 13:35
Last Modified:28 Apr 2024 01:47
Publisher:Elsevier
ISSN:0027-5107
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/0027-5107(69)90053-0
PubMed ID:5801080
Other Identification Number:Corpus ID: 30136319