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Pre-implantation embryos of chinese hamster. II. Incidence and type of karyotype anomalies after treatment of the paternal post-meiotic germ cells with an alkylating mutagen


Binkert, Franz; Schmid, Werner (1977). Pre-implantation embryos of chinese hamster. II. Incidence and type of karyotype anomalies after treatment of the paternal post-meiotic germ cells with an alkylating mutagen. Mutation Research - Section on Environmental Mutagenesis and Related Subjects, 46(2):77-86.

Abstract

Ninety-two male Chinese hamsters were treated with a single, sub-lethal dose of the alkylating cytostatic drug Trenimon. After 3--23 days they were mated with untreated females. The great majority of the male germ cells had been exposed to the mutagen while they were in the highly sensitive post-meiotic spermatid stage. The karyotypes of the resulting embryos were studied in the 4--8-cell stage. Out of 221 analysable embryos, 24.4% had aberrant karyotypes. Ploidy and genome mutations were, at 0.9% each, within control limits. Structural aberrations, involving one or several chromosomes, were present in 23.6% of the embryos (control 1.8%). 51% had a single aberrant centric element. The most frequent aberration types were deletions (54%), dicentrics (16%), translocations inversions and complex rearrangements with 22% and rings with 7%. About one-third of the cells, in addition, contained acentric fragments.

Abstract

Ninety-two male Chinese hamsters were treated with a single, sub-lethal dose of the alkylating cytostatic drug Trenimon. After 3--23 days they were mated with untreated females. The great majority of the male germ cells had been exposed to the mutagen while they were in the highly sensitive post-meiotic spermatid stage. The karyotypes of the resulting embryos were studied in the 4--8-cell stage. Out of 221 analysable embryos, 24.4% had aberrant karyotypes. Ploidy and genome mutations were, at 0.9% each, within control limits. Structural aberrations, involving one or several chromosomes, were present in 23.6% of the embryos (control 1.8%). 51% had a single aberrant centric element. The most frequent aberration types were deletions (54%), dicentrics (16%), translocations inversions and complex rearrangements with 22% and rings with 7%. About one-third of the cells, in addition, contained acentric fragments.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Genetics
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > Toxicology
Life Sciences > Genetics
Uncontrolled Keywords:Genetics, Toxicology
Language:English
Date:1 April 1977
Deposited On:27 Jan 2023 12:30
Last Modified:28 Apr 2024 01:47
Publisher:Elsevier
ISSN:0165-1161
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/0165-1161(77)90114-5
PubMed ID:876266
Other Identification Number:Corpus ID: 24586401