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Common Presence of Phototrophic Gemmatimonadota in Temperate Freshwater Lakes

Mujakić, Izabela; Andrei, Adrian-Stefan; Shabarova, Tanja; Fecskeová, Lívia Kolesár; Salcher, Michaela M; Piwosz, Kasia; Ghai, Rohit; Koblížek, Michal (2021). Common Presence of Phototrophic Gemmatimonadota in Temperate Freshwater Lakes. mSystems, 6(2):01241-20.

Abstract

Members of the bacterial phylum Gemmatimonadota are ubiquitous in most natural environments and represent one of the top 10 most abundant bacterial phyla in soil. Sequences affiliated with Gemmatimonadota were also reported from diverse aquatic habitats; however, it remains unknown whether they are native organisms or represent bacteria passively transported from sediment or soil. To address this question, we analyzed metagenomes constructed from five freshwater lakes in central Europe. Based on the 16S rRNA gene frequency, Gemmatimonadota represented from 0.02 to 0.6% of all bacteria in the epilimnion and between 0.1 and 1% in the hypolimnion. These proportions were independently confirmed using catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). Some cells in the epilimnion were attached to diatoms (Fragilaria sp.) or cyanobacteria (Microcystis sp.), which suggests a close association with phytoplankton. In addition, we reconstructed 45 metagenome-assembled genomes (MAGs) related to Gemmatimonadota. They represent several novel lineages, which persist in the studied lakes during the seasons. Three lineages contained photosynthesis gene clusters. One of these lineages was related to Gemmatimonas phototrophica and represented the majority of Gemmatimonadota retrieved from the lakes’ epilimnion. The other two lineages came from hypolimnion and probably represented novel photoheterotrophic genera. None of these phototrophic MAGs contained genes for carbon fixation. Since most of the identified MAGs were present during the whole year and cells associated with phytoplankton were observed, we conclude that they represent truly limnic Gemmatimonadota distinct from the previously described species isolated from soils or sediments.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
Dewey Decimal Classification:580 Plants (Botany)
Scopus Subject Areas:Life Sciences > Microbiology
Life Sciences > Ecology, Evolution, Behavior and Systematics
Life Sciences > Biochemistry
Life Sciences > Physiology
Physical Sciences > Modeling and Simulation
Life Sciences > Molecular Biology
Life Sciences > Genetics
Physical Sciences > Computer Science Applications
Language:English
Date:2021
Deposited On:24 Feb 2023 07:24
Last Modified:29 Oct 2024 02:38
Publisher:American Society for Microbiology
ISSN:2379-5077
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1128/mSystems.01241-20
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  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)

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