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Kidney capsule transplantation of chemically treated embryonic murine tooth germs


Jiménez-Rojo, Lucia; Mitsiadis, Thimios A (2023). Kidney capsule transplantation of chemically treated embryonic murine tooth germs. STAR Protocols, 4(3):102377.

Abstract

Subcapsular transplantation of developing tissues and organs into the richly vascularized murine kidney provides the necessary trophic support, thus ensuring proper completion of their growth. Here, we provide a protocol for kidney capsule transplantation that allows the full differentiation of embryonic teeth previously exposed to chemicals. We describe steps for dissection and in vitro culture of embryonic teeth, followed by transplantation of tooth germs. We then detail harvesting of kidneys for further analysis. For complete details on the use and execution of this protocol, please refer to Mitsiadis et al.

Abstract

Subcapsular transplantation of developing tissues and organs into the richly vascularized murine kidney provides the necessary trophic support, thus ensuring proper completion of their growth. Here, we provide a protocol for kidney capsule transplantation that allows the full differentiation of embryonic teeth previously exposed to chemicals. We describe steps for dissection and in vitro culture of embryonic teeth, followed by transplantation of tooth germs. We then detail harvesting of kidneys for further analysis. For complete details on the use and execution of this protocol, please refer to Mitsiadis et al.

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Additional indexing

Item Type:Journal Article, refereed, further contribution
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Institute of Oral Biology
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Life Sciences > General Neuroscience
Life Sciences > General Biochemistry, Genetics and Molecular Biology
Life Sciences > General Immunology and Microbiology
Uncontrolled Keywords:Cell Culture; Cell Differentiation; Developmental Biology; Model Organisms
Language:English
Date:1 September 2023
Deposited On:06 Nov 2023 13:57
Last Modified:29 Apr 2024 01:40
Publisher:Cell Press (Elsevier)
ISSN:2666-1667
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/j.xpro.2023.102377
PubMed ID:37379218
  • Content: Published Version
  • Language: English
  • Licence: Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)