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Dynamics of transcriptional programs and chromatin accessibility in mouse spermatogonial cells from early postnatal to adult life


Lazar-Contes, Irina; Tanwar, Deepak K; Arzate-Mejia, Rodrigo G; Steg, Leonard C; Feudjio, Olivier Ulrich; Crespo, Marion; Germain, Pierre-Luc; Mansuy, Isabelle M (2023). Dynamics of transcriptional programs and chromatin accessibility in mouse spermatogonial cells from early postnatal to adult life. eLife:Epub ahead of print.

Abstract

In mammals, spermatogonial cells (SCs) are undifferentiated male germ cells in testis quiescent until birth that self-renew and differentiate to produce spermatogenic cells and functional sperm across life. The transcriptome of SCs is highly dynamic and timely regulated during postnatal development. We examined if such dynamics involves changes in chromatin organization by profiling the transcriptome and chromatin accessibility in SCs from early postnatal stages to adulthood in mice using RNA-seq and ATAC-seq. By integrating transcriptomic and epigenomic features, we show that SCs undergo massive chromatin remodeling during postnatal development that correlates with distinct gene expression profiles and transcription factors (TF) motif enrichment. We identify genomic regions with significantly different chromatin accessibility in adult SCs that are marked by histone modifications associated with enhancers and promoters. Some of the regions with increased accessibility correspond to transposable element subtypes enriched in multiple TFs motifs and close to differentially expressed genes. Our results underscore the dynamics of chromatin organization in developing germ cells and the involvement of the regulatory genome.

Abstract

In mammals, spermatogonial cells (SCs) are undifferentiated male germ cells in testis quiescent until birth that self-renew and differentiate to produce spermatogenic cells and functional sperm across life. The transcriptome of SCs is highly dynamic and timely regulated during postnatal development. We examined if such dynamics involves changes in chromatin organization by profiling the transcriptome and chromatin accessibility in SCs from early postnatal stages to adulthood in mice using RNA-seq and ATAC-seq. By integrating transcriptomic and epigenomic features, we show that SCs undergo massive chromatin remodeling during postnatal development that correlates with distinct gene expression profiles and transcription factors (TF) motif enrichment. We identify genomic regions with significantly different chromatin accessibility in adult SCs that are marked by histone modifications associated with enhancers and promoters. Some of the regions with increased accessibility correspond to transposable element subtypes enriched in multiple TFs motifs and close to differentially expressed genes. Our results underscore the dynamics of chromatin organization in developing germ cells and the involvement of the regulatory genome.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Brain Research Institute
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:22 November 2023
Deposited On:16 Jan 2024 15:03
Last Modified:17 Jan 2024 09:57
Publisher:eLife Sciences Publications Ltd.
ISSN:2050-084X
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.7554/elife.91528.1
  • Content: Published Version
  • Language: English
  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)