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A gravity‐independent single‐phase electrode reservoir for capillary electrophoresis applications


Drevinskas, Tomas; Noell, Aaron C; Kehl, Florian; Zamuruyev, Konstantin; Ferreira Santos, Mauro S; Mora, Maria F; Boone, Travis D; Hoac, Trinh; Quinn, Richard C; Ricco, Antonio J; Willis, Peter A (2023). A gravity‐independent single‐phase electrode reservoir for capillary electrophoresis applications. Electrophoresis, 44(13-14):1047-1056.

Abstract

Capillary electrophoresis (CE) holds great promise as an in situ analytical technique for a variety of applications. However, typical instrumentation operates with open reservoirs (e.g., vials) to accommodate reagents and samples, which is problematic for automated instruments designed for space or underwater applications that may be operated in various orientations. Microgravity conditions add an additional challenge due to the unpredictable position of the headspace (air layer above the liquid) in any two‐phase reservoir. One potential solution for these applications is to use a headspace‐free, flow‐through reservoir design that is sealed and connected to the necessary reagents and samples. Here, we demonstrate a flow‐through high‐voltage (HV) reservoir for CE that is compatible with automated in situ exploration needs, and which can be electrically isolated from its source fluidics (in order to prevent unwanted leakage current). We also demonstrate how the overall system can be rationally designed based on the operational parameters for CE to prevent electrolysis products generated at the electrode from entering the capillary and interfering with the CE separation. A reservoir was demonstrated with a 19 mm long, 1.8 mm inner diameter channel connecting the separation capillary and the HV electrode. Tests of these reservoirs integrated into a CE system show reproducible CE system operation with a variety of background electrolytes at voltages up to 25 kV. Rotation of the reservoirs, and the system, showed that their performance was independent of the direction of the gravity vector.

Abstract

Capillary electrophoresis (CE) holds great promise as an in situ analytical technique for a variety of applications. However, typical instrumentation operates with open reservoirs (e.g., vials) to accommodate reagents and samples, which is problematic for automated instruments designed for space or underwater applications that may be operated in various orientations. Microgravity conditions add an additional challenge due to the unpredictable position of the headspace (air layer above the liquid) in any two‐phase reservoir. One potential solution for these applications is to use a headspace‐free, flow‐through reservoir design that is sealed and connected to the necessary reagents and samples. Here, we demonstrate a flow‐through high‐voltage (HV) reservoir for CE that is compatible with automated in situ exploration needs, and which can be electrically isolated from its source fluidics (in order to prevent unwanted leakage current). We also demonstrate how the overall system can be rationally designed based on the operational parameters for CE to prevent electrolysis products generated at the electrode from entering the capillary and interfering with the CE separation. A reservoir was demonstrated with a 19 mm long, 1.8 mm inner diameter channel connecting the separation capillary and the HV electrode. Tests of these reservoirs integrated into a CE system show reproducible CE system operation with a variety of background electrolytes at voltages up to 25 kV. Rotation of the reservoirs, and the system, showed that their performance was independent of the direction of the gravity vector.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute for Computational Science
04 Faculty of Medicine > Institute of Anatomy
Dewey Decimal Classification:530 Physics
570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > Biochemistry
Life Sciences > Clinical Biochemistry
Uncontrolled Keywords:Clinical Biochemistry, Biochemistry, Analytical Chemistry
Language:English
Date:1 July 2023
Deposited On:02 Feb 2024 17:17
Last Modified:31 May 2024 01:52
Publisher:Wiley-VCH Verlag
ISSN:0173-0835
OA Status:Closed
Publisher DOI:https://doi.org/10.1002/elps.202300015
PubMed ID:36966381
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