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A novel method for automated crystal visualization and quantification in murine folic acid-induced acute kidney injury


Hamid, Ahmad Kamal; Pastor Arroyo, Eva Maria; Lee, Sung Sik; Wagner, Carsten Alexander; Egli-Spichtig, Daniela (2024). A novel method for automated crystal visualization and quantification in murine folic acid-induced acute kidney injury. American Journal of Physiology : Renal Physiology, 326(1):F105-F117.

Abstract

Here, we describe a novel method for the visualization and quantification of renal folic acid (FA) crystals in the rodent FA-induced acute kidney injury (FA-AKI) model. The protocol involves a straightforward histological approach followed by fully automated imaging and quantification steps. Applicability was confirmed by showing that the FA-AKI model is sex-dependent. The method can serve as a tool to aid in characterizing FA-AKI and to control for studies investigating prophylactic therapeutic avenues using FA-AKI.

Abstract

Here, we describe a novel method for the visualization and quantification of renal folic acid (FA) crystals in the rodent FA-induced acute kidney injury (FA-AKI) model. The protocol involves a straightforward histological approach followed by fully automated imaging and quantification steps. Applicability was confirmed by showing that the FA-AKI model is sex-dependent. The method can serve as a tool to aid in characterizing FA-AKI and to control for studies investigating prophylactic therapeutic avenues using FA-AKI.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
Dewey Decimal Classification:610 Medicine & health
570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Physiology
Health Sciences > Urology
Uncontrolled Keywords:Physiology
Language:English
Date:1 January 2024
Deposited On:08 Feb 2024 10:28
Last Modified:30 Jun 2024 01:39
Publisher:American Physiological Society
ISSN:1522-1466
OA Status:Hybrid
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1152/ajprenal.00140.2023
PubMed ID:37881875
Project Information:
  • : FunderGottfried und Julia Bangerter-Rhyner-Stiftung
  • : Grant ID
  • : Project Title
  • : FunderOlga Mayenfisch Stiftung
  • : Grant ID
  • : Project Title
  • : FunderSNF | Swiss National Centre of Competence in Research Kidney Control of Homeostasis
  • : Grant ID
  • : Project Title
  • Content: Published Version
  • Language: English
  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)
  • Content: Accepted Version
  • Language: English