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Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin

Tyson, Jess; Radford, Paul; Lambert, Carey; Till, Rob; Huwiler, Simona G; Lovering, Andrew L; Sockett, R Elizabeth (2024). Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin. Nature Communications, 15(1):3078.

Abstract

The bacterium Bdellovibrio bacteriovorus is a predator of other Gram-negative bacteria. The predator invades the prey’s periplasm and modifies the prey’s cell wall, forming a rounded killed prey, or bdelloplast, containing a live B. bacteriovorus. Redundancy in adhesive processes makes invasive mutants rare. Here, we identify a MIDAS adhesin family protein, Bd0875, that is expressed at the predator-prey invasive junction and is important for successful invasion of prey. A mutant strain lacking bd0875 is still able to form round, dead bdelloplasts; however, 10% of the bdelloplasts do not contain B. bacteriovorus, indicative of an invasion defect. Bd0875 activity requires the conserved MIDAS motif, which is linked to catch-and-release activity of MIDAS proteins in other organisms. A proteomic analysis shows that the uninvaded bdelloplasts contain B. bacteriovorus proteins, which are likely secreted into the prey by the Δbd0875 predator during an abortive invasion period. Thus, secretion of proteins into the prey seems to be sufficient for prey killing, even in the absence of a live predator inside the prey periplasm.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
Dewey Decimal Classification:580 Plants (Botany)
Scopus Subject Areas:Physical Sciences > General Chemistry
Life Sciences > General Biochemistry, Genetics and Molecular Biology
Physical Sciences > General Physics and Astronomy
Uncontrolled Keywords:Microbiology, Predatory bacteria, Bdellovibrio bacteriovorus, Genetics and Molecular Biology,
Scope:Discipline-based scholarship (basic research)
Language:English
Date:9 April 2024
Deposited On:23 Apr 2024 11:08
Last Modified:27 Feb 2025 02:44
Publisher:Nature Publishing Group
ISSN:2041-1723
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1038/s41467-024-47412-3
PubMed ID:38594280
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  • Content: Published Version
  • Language: English
  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)

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