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γ1 GABA$_{A}$Receptors in Spinal Nociceptive Circuits

Neumann, Elena; Cramer, Teresa; Acuña, Mario A; Scheurer, Louis; Beccarini, Camilla; Luscher, Bernhard; Wildner, Hendrik; Zeilhofer, Hanns Ulrich (2024). γ1 GABA$_{A}$Receptors in Spinal Nociceptive Circuits. Journal of Neuroscience, 44(41):e0591242024.

Abstract

GABAergic neurons and GABA$_{A}$receptors (GABA$_{A}$Rs) are critical elements of almost all neuronal circuits. Most GABA$_{A}$Rs of the CNS are heteropentameric ion channels composed of two α, two β, and one γ subunits. These receptors serve as important drug targets for benzodiazepine (BDZ) site agonists, which potentiate the action of GABA at GABA$_{A}$Rs. Most GABA$_{A}$R classifications rely on the heterogeneity of the α subunit (α1–α6) included in the receptor complex. Heterogeneity of the γ subunits (γ1–γ3), which mediate synaptic clustering of GABA$_{A}$Rs and contribute, together with α subunits, to the benzodiazepine (BDZ) binding site, has gained less attention, mainly because γ2 subunits greatly outnumber the other γ subunits in most brain regions. Here, we have investigated a potential role of non-γ2 GABA$_{A}$Rs in neural circuits of the spinal dorsal horn, a key site of nociceptive processing. Female and male mice were studied. We demonstrate that besides γ2 subunits, γ1 subunits are significantly expressed in the spinal dorsal horn, especially in its superficial layers. Unlike global γ2 subunit deletion, which is lethal, spinal cord-specific loss of γ2 subunits was well tolerated. GABA$_{A}$R clustering in the superficial dorsal horn remained largely unaffected and antihyperalgesic actions of HZ-166, a nonsedative BDZ site agonist, were partially retained. Our results thus suggest that the superficial dorsal horn harbors functionally relevant amounts of γ1 subunits that support the synaptic clustering of GABA$_{A}$Rs in this site. They further suggest that γ1 containing GABA$_{A}$Rs contribute to the spinal control of nociceptive information flow.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > General Neuroscience
Language:English
Date:9 October 2024
Deposited On:18 Oct 2024 05:43
Last Modified:19 Oct 2024 20:00
Publisher:Society for Neuroscience
ISSN:0270-6474
OA Status:Hybrid
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1523/jneurosci.0591-24.2024
PubMed ID:39137998
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  • Language: English
  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)

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