Header

UZH-Logo

Maintenance Infos

Distribution of macrophages and T cells in syngrafts and allografts after experimental rat lung transplantation


Jungraithmayr, W; Inci, I; Bain, M; Hillinger, S; Korom, S; Weder, W (2010). Distribution of macrophages and T cells in syngrafts and allografts after experimental rat lung transplantation. Immunobiology, 215(3):206-214.

Abstract

Macrophages and T cells have a pivotal role in orchestrating the acute lung allograft rejection response. We investigated the spatial and temporal distribution of these immune cells and the synthesis patterns of the T(h)1- and T(h)2-cytokine IL-12 and IL-10 during the early course after transplantation (Tx). Orthotopic single-lung Tx was performed in Lewis to Lewis (syngrafts) and Brown Norway/Lewis F(1) hybrid to Lewis (allografts). Naïve lungs, syngrafts after 5 days and allografts after 3 and 5 days were analyzed for CD68(+), CD163(+) and CD3(+) cells by immunohistochemistry and IL-12 and IL-10 were detected by immunofluorescence. CD68(+) macrophages increased in number after allogeneic Tx compared to syngeneic Tx on day 5 (P<.001), CD163(+) macrophages sequestrated early around veins (day 3) compared to the accumulation around arteries and bronchioles (P<.001) while CD3(+) T cells were scarce. There was a predominance of IL-12 over IL-10 on day 5 after allogeneic Tx (P<.001). CD68(+) macrophages were the most abundant cells during acute pulmonary rejection and CD163(+) macrophages showed a characteristic distribution pattern over time around vessels and bronchioles. The up-regulation of IL-12 reflects an early response after allo-antigen exposure, indicating a strong impact of the initiation of the T(h)1 pathway at an early phase during acute lung rejection.

Abstract

Macrophages and T cells have a pivotal role in orchestrating the acute lung allograft rejection response. We investigated the spatial and temporal distribution of these immune cells and the synthesis patterns of the T(h)1- and T(h)2-cytokine IL-12 and IL-10 during the early course after transplantation (Tx). Orthotopic single-lung Tx was performed in Lewis to Lewis (syngrafts) and Brown Norway/Lewis F(1) hybrid to Lewis (allografts). Naïve lungs, syngrafts after 5 days and allografts after 3 and 5 days were analyzed for CD68(+), CD163(+) and CD3(+) cells by immunohistochemistry and IL-12 and IL-10 were detected by immunofluorescence. CD68(+) macrophages increased in number after allogeneic Tx compared to syngeneic Tx on day 5 (P<.001), CD163(+) macrophages sequestrated early around veins (day 3) compared to the accumulation around arteries and bronchioles (P<.001) while CD3(+) T cells were scarce. There was a predominance of IL-12 over IL-10 on day 5 after allogeneic Tx (P<.001). CD68(+) macrophages were the most abundant cells during acute pulmonary rejection and CD163(+) macrophages showed a characteristic distribution pattern over time around vessels and bronchioles. The up-regulation of IL-12 reflects an early response after allo-antigen exposure, indicating a strong impact of the initiation of the T(h)1 pathway at an early phase during acute lung rejection.

Statistics

Citations

Dimensions.ai Metrics
5 citations in Web of Science®
3 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

1 download since deposited on 12 Feb 2010
0 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Thoracic Surgery
04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Health Sciences > Immunology and Allergy
Life Sciences > Immunology
Health Sciences > Hematology
Language:English
Date:March 2010
Deposited On:12 Feb 2010 14:43
Last Modified:23 Jan 2022 16:00
Publisher:Elsevier
ISSN:0171-2985
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.imbio.2009.04.003
PubMed ID:19457578