The spectral reflectance of tree crown can be different from spectral reflectance of its leaves because of diverse leaf and branch angles as well as internal space of tree crown. For these reasons it is necessary to study spectral reflectance of both adaxial and abaxial surfaces of the leaves. Such information is necessity for modeling the reflectance of tree crown and forest stands. The main objective of this study was to obtain and study the spectral reflectance of both adaxial and abaxial leaves of beech and hornbeam in natural condition and to investigate their spectral differences using indices sensitive to chlorophyll, chlorophyll to carotenoid ratio and photosynthetic pigments. Field spectroradiometric measurements were performed using a portable spectroradiometer (ASD FieldSpec) in Kheyrud forest. A total of 52 trees were sampled and 312 spectra were recorded and analyzed. Spectral measurements cover the wavelength range between 350 – 2500 nm. The results of the spectral reflectance analysis of these two species showed that the abaxial spectral reflectance from 350 to 2500 nm was higher than the adaxial one for hornbeam species. However, for beech species in the visible region and far infrared region, the abaxial spectral reflectance was higher whereas in the near infrared it was lower than the adaxial one. For more detailed investigation of spectral reflectance difference for these two species, spectral indices sensitive to chlorophyll and carotenoid were calculated and statistically analyzed for both surfaces. The value of adaxial NDI index was found to be higher than abaxial for both species. In contrast, the values of adaxial SIPI and PRI indices were lower than abaxial. The differences significant (?= 0.01, p< 0.0001) for both species.