Header

UZH-Logo

Maintenance Infos

Development and application of a real-time TaqMan® qPCR assay for detection and quantification of 'Candidatus Mycoplasma haemolamae' in South American camelids


Meli, Marina L; Kaufmann, C; Zanolari, P; Robert, N; Willi, Barbara; Lutz, H; Hofmann-Lehmann, R (2010). Development and application of a real-time TaqMan® qPCR assay for detection and quantification of 'Candidatus Mycoplasma haemolamae' in South American camelids. Veterinary Microbiology, 146(3-4):209-294.

Abstract

Two alpacas from a herd in southwest Switzerland died for unknown reasons. Necropsy revealed chronic weight loss and pale mucous membranes. Infection with hemotropic mycoplasmas was suspected and subsequently confirmed by molecular methods. In order to investigate the epidemiological situation in this herd, a real-time TaqMan(®) qPCR assay for the specific detection and quantification of hemoplasma infection in South American camelids was developed. This assay was based on the 16S rRNA gene and amplified 'Candidatus Mycoplasma haemolamae' DNA, but not DNA from other hemoplasmas or non-hemotropic mycoplasma species. The lower detection limit was one copy/PCR, and the amplification efficiency was 97.4%. In 11 out of 24 clinically healthy herd mates of the two infected alpacas, 'Candidatus M. haemolamae' infection was confirmed. No correlation was found between bacterial load and clinical signs or anemia. The assay described herein enables to detect and quantify 'Candidatus M. haemolamae' and may be used in future studies to investigate the prevalence, pathogenesis and treatment follow-up of hemoplasma infections in South American camelids.

Abstract

Two alpacas from a herd in southwest Switzerland died for unknown reasons. Necropsy revealed chronic weight loss and pale mucous membranes. Infection with hemotropic mycoplasmas was suspected and subsequently confirmed by molecular methods. In order to investigate the epidemiological situation in this herd, a real-time TaqMan(®) qPCR assay for the specific detection and quantification of hemoplasma infection in South American camelids was developed. This assay was based on the 16S rRNA gene and amplified 'Candidatus Mycoplasma haemolamae' DNA, but not DNA from other hemoplasmas or non-hemotropic mycoplasma species. The lower detection limit was one copy/PCR, and the amplification efficiency was 97.4%. In 11 out of 24 clinically healthy herd mates of the two infected alpacas, 'Candidatus M. haemolamae' infection was confirmed. No correlation was found between bacterial load and clinical signs or anemia. The assay described herein enables to detect and quantify 'Candidatus M. haemolamae' and may be used in future studies to investigate the prevalence, pathogenesis and treatment follow-up of hemoplasma infections in South American camelids.

Statistics

Citations

Dimensions.ai Metrics
9 citations in Web of Science®
10 citations in Scopus®
6 citations in Microsoft Academic
Google Scholar™

Altmetrics

Downloads

0 downloads since deposited on 18 Jan 2011
0 downloads since 12 months

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Department of Small Animals
05 Vetsuisse Faculty > Veterinary Clinic > Department of Farm Animals
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Scopus Subject Areas:Life Sciences > Microbiology
Health Sciences > General Veterinary
Uncontrolled Keywords:Molecular diagnostics
Language:English
Date:2010
Deposited On:18 Jan 2011 15:07
Last Modified:29 Jul 2020 23:16
Publisher:Elsevier
ISSN:0378-1135
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.vetmic.2010.05.029
PubMed ID:21095509

Download

Closed Access: Download allowed only for UZH members