Header

UZH-Logo

Maintenance Infos

Miniaturized selective plane illumination microscopy for high-contrast in vivo fluorescence imaging


Engelbrecht, C E; Voigt, F; Helmchen, F (2010). Miniaturized selective plane illumination microscopy for high-contrast in vivo fluorescence imaging. Optics Letters, 35(9):1413-1415.

Abstract

Light-sheet-based fluorescence imaging techniques rely on simultaneous excitation of a single optical plane and thus permit high-contrast optically sectioned imaging of extended tissue samples. Here, we introduce a miniaturized fiber-optic implementation of a selective plane-illumination microscope (miniSPIM). The excitation light was delivered through a single-mode optical fiber, and a light-sheet was created with a cylindrical gradient-index lens and a right-angle microprism. Fluorescence emission was collected orthogonally to the light-sheet through a gradient-index lens assembly and a coherent fiber bundle. The end face of the fiber bundle was imaged onto a charge-coupled device camera. The spatial resolutions of the miniSPIM were 3.2 μm laterally and 5.1 μm axially. Images of fluorescent beads and neurons in mouse neocortex exhibited superior axial resolution and contrast in the miniSPIM-mode compared to images recorded in epi-illumination mode. The miniSPIM may thus enable novel in vivo imaging approaches.

Abstract

Light-sheet-based fluorescence imaging techniques rely on simultaneous excitation of a single optical plane and thus permit high-contrast optically sectioned imaging of extended tissue samples. Here, we introduce a miniaturized fiber-optic implementation of a selective plane-illumination microscope (miniSPIM). The excitation light was delivered through a single-mode optical fiber, and a light-sheet was created with a cylindrical gradient-index lens and a right-angle microprism. Fluorescence emission was collected orthogonally to the light-sheet through a gradient-index lens assembly and a coherent fiber bundle. The end face of the fiber bundle was imaged onto a charge-coupled device camera. The spatial resolutions of the miniSPIM were 3.2 μm laterally and 5.1 μm axially. Images of fluorescent beads and neurons in mouse neocortex exhibited superior axial resolution and contrast in the miniSPIM-mode compared to images recorded in epi-illumination mode. The miniSPIM may thus enable novel in vivo imaging approaches.

Statistics

Citations

Dimensions.ai Metrics
19 citations in Web of Science®
21 citations in Scopus®
26 citations in Microsoft Academic
Google Scholar™

Altmetrics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Brain Research Institute
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2010
Deposited On:04 Jan 2011 09:57
Last Modified:17 Aug 2018 21:59
Publisher:Optical Society of America (OSA)
ISSN:0146-9592
OA Status:Closed
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1364/OL.35.001413
PubMed ID:20436587
Project Information:
  • : FunderFP7
  • : Grant ID200873
  • : Project TitleSPACEBRAIN - Space coding in hippocampo-entorhinal neuronal assemblies

Download

Full text not available from this repository.
View at publisher

Get full-text in a library