Objectives: The aim of this study was to test the impact of Gluma Desensitizer on long-term shear bond strength of two self-adhesive resin cements. Methods: Molars (N=550) were embedded in acrylic resin and cemented with: i) Panavia21 (control group), ii) RelyX Unicem, iii) RelyX Unicem combined with Gluma Desensitizer, iv) G-Cem, and v) G-Cem combined with Gluma Desensitizer. After the initial shear bond strength of all the groups were measured, half of the remaining specimens were stored in water and the other half in thermocycling (1d, 4d, 9d, 16d and 25d). The shear bond strength was measured in a Universal Testing Machine (1 mm/min, Zwick Z010). Data were analyzed with one-way ANOVA followed by a post-hoc Scheffé test and t-test (p<0.05). Results: Overall, both self-adhesive resin cements combined with Gluma Desensitizer produced higher shear bond strength under all tested conditions compared to the control group, and to the self-adhesive resin cements without Gluma Desensitizer. The two aging types showed ambigious results. Significance: The application of the Gluma Desensitizer shows positive aging effect on shear bond strength.