Prevalence of Chlamydophila psittaci in wild birds - potential risk for domestic poultry, pet birds and public health?

Abstract

To determine the prevalence of Chlamydophila (C.) psittaci in wild birds, cloacal swabs from 527 songbirds, 442 waterfowl, 84 feral pigeons and 38 cormorants were examined by Chlamydiaceae-specific real-time polymerase chain reaction (PCR) and ArrayTube microarray assays for chlamydial species determination and genotyping of C. psittaci. Inconclusive cases were further characterized by conventional PCR methods targeting the chlamydial outer membrane protein A, 16S, 23S, and intrgenic spacer genes followed by sequencing of the PCR product. Swabs of 19 water birds (tufted ducks and pochards), 12 pigeons and one songbird were tested positive by the Chlamydiaceae-specific real-time PCR. While C. psittaci genotypes B (n=5) and E (n=1) were identified in feral pigeons (n=9), the genotype could not be identified in the remaining three cases. Sequence data of Chlamydiaceae-positive cases (n=23; 19 waterfowl, three pigeons, one songbird) indicated the presence of non-classified chlamydial agents (n=20) and C. psittaci (n=3) by 16S rRNA PCR and sequencing. In conclusion, C. psittaci was not detected in waterfowl and songbirds, but C. psittaci proved prevalent in urban feral pigeons, where it poses a significant risk for humans.