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The development of microsatellite loci in the song sparrow, Melospiza melodia (Aves) and genotyping errors associated with good quality DNA


Jeffery, K J; Keller, L F; Arcese, P; Bruford, M W (2001). The development of microsatellite loci in the song sparrow, Melospiza melodia (Aves) and genotyping errors associated with good quality DNA. Molecular Ecology Notes, 1(1-2):11-13.

Abstract

We describe the isolation, development and application of six microsatellite loci in the song sparrow, Melospiza melodia, their amplification in related species and problems encountered in genotyping from high quality DNA. Heterozygosity in an island song sparrow population was high (> 70%) for five out of six loci. Small scale cross-species amplification showed up to three alleles per locus for other Emberizidae. Relatively high levels of allelic dropout were observed with two loci when using standard template DNA. These problems could be partially alleviated by diluting template DNA, and could always be eliminated by repeated genotyping.

Abstract

We describe the isolation, development and application of six microsatellite loci in the song sparrow, Melospiza melodia, their amplification in related species and problems encountered in genotyping from high quality DNA. Heterozygosity in an island song sparrow population was high (> 70%) for five out of six loci. Small scale cross-species amplification showed up to three alleles per locus for other Emberizidae. Relatively high levels of allelic dropout were observed with two loci when using standard template DNA. These problems could be partially alleviated by diluting template DNA, and could always be eliminated by repeated genotyping.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
Dewey Decimal Classification:570 Life sciences; biology
590 Animals (Zoology)
Scopus Subject Areas:Life Sciences > Biochemistry
Physical Sciences > Ecology
Life Sciences > General Biochemistry, Genetics and Molecular Biology
Language:English
Date:March 2001
Deposited On:27 Apr 2012 07:33
Last Modified:30 Jul 2020 02:38
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN:1471-8286
OA Status:Closed
Publisher DOI:https://doi.org/10.1046/j.1471-8278 .2000.00005.x
Other Identification Number:ISI:000174289500006

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