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Chronic imaging of cortical sensory map dynamics using a genetically encoded calcium indicator


Minderer, M; Liu, W; Sumanovski, L T; Kügler, S; Helmchen, F; Margolis, D J (2012). Chronic imaging of cortical sensory map dynamics using a genetically encoded calcium indicator. Journal of Physiology, 590(1):99-107.

Abstract

Abstract  In vivo optical imaging can reveal the dynamics of large-scale cortical activity, but methods for chronic recording are limited. Here we present a technique for long-term investigation of cortical map dynamics using wide-field ratiometric fluorescence imaging of the genetically encoded calcium indicator (GECI) Yellow Cameleon 3.60. We find that wide-field GECI signals report sensory-evoked activity in anaesthetized mouse somatosensory cortex with high sensitivity and spatiotemporal precision, and furthermore, can be measured repeatedly in separate imaging sessions over multiple weeks. This method opens new possibilities for the longitudinal study of stability and plasticity of cortical sensory representations.

Abstract

Abstract  In vivo optical imaging can reveal the dynamics of large-scale cortical activity, but methods for chronic recording are limited. Here we present a technique for long-term investigation of cortical map dynamics using wide-field ratiometric fluorescence imaging of the genetically encoded calcium indicator (GECI) Yellow Cameleon 3.60. We find that wide-field GECI signals report sensory-evoked activity in anaesthetized mouse somatosensory cortex with high sensitivity and spatiotemporal precision, and furthermore, can be measured repeatedly in separate imaging sessions over multiple weeks. This method opens new possibilities for the longitudinal study of stability and plasticity of cortical sensory representations.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Brain Research Institute
Special Collections > SystemsX.ch > Research, Technology and Development Projects > Neurochoice
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2012
Deposited On:11 Jan 2012 12:58
Last Modified:20 Sep 2018 07:38
Publisher:Wiley-Blackwell
ISSN:0022-3751
OA Status:Closed
Publisher DOI:https://doi.org/10.1113/jphysiol.2011.219014
PubMed ID:22083602
Project Information:
  • : FunderFP7
  • : Grant ID243914
  • : Project TitleBRAIN-I-NETS - Novel Brain-Inspired Learning Paradigms for Large-Scale Neuronal Networks
  • : FunderFP7
  • : Grant ID223524
  • : Project TitlePLASTICISE - Promotion of plasticity as a treatment for neurodegenerative conditions

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