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Glycoprotein maturation and the UPR


Hülsmeier, A J; Welti, M; Hennet, T (2011). Glycoprotein maturation and the UPR. In: Conn, P M. The unfolded protein response and cellular stress. Part C. Amsterdam, NL: Elsevier, 163-82.

Abstract

Glycosylation is a complex form of protein modification occurring in the secretory pathway. The addition of N- and O-glycans affects intracellular processes like the folding and trafficking of most glycoproteins. To better understand the impact of glycosylation in protein folding and maturation, parameters like glycosylation site occupancy and oligosaccharide structure must be measured quantitatively. In this chapter, we describe current methods enabling the determination of N-glycosylation by assessment of cellular dolichol phosphate levels, dolichol-linked oligosaccharides, and the occupancy of N-glycosylation sites. We also provide detailed methods for the analysis of O-glycosylation, whose role in intracellular protein maturation is often overlooked.

Abstract

Glycosylation is a complex form of protein modification occurring in the secretory pathway. The addition of N- and O-glycans affects intracellular processes like the folding and trafficking of most glycoproteins. To better understand the impact of glycosylation in protein folding and maturation, parameters like glycosylation site occupancy and oligosaccharide structure must be measured quantitatively. In this chapter, we describe current methods enabling the determination of N-glycosylation by assessment of cellular dolichol phosphate levels, dolichol-linked oligosaccharides, and the occupancy of N-glycosylation sites. We also provide detailed methods for the analysis of O-glycosylation, whose role in intracellular protein maturation is often overlooked.

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Additional indexing

Item Type:Book Section, refereed, further contribution
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Biochemistry
Life Sciences > Molecular Biology
Language:English
Date:2011
Deposited On:27 Jan 2012 21:46
Last Modified:23 Jan 2022 20:15
Publisher:Elsevier
Series Name:Methods in Enzymology
ISSN:0076-6879 (P) 1557-7988 (E)
ISBN:978-0-12-385928-0
OA Status:Green
Publisher DOI:https://doi.org/10.1016/B978-0-12-385928-0.00010-9
PubMed ID:21329800
  • Content: Accepted Version
  • Language: English