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Evaluation of three commercially available real-time PCR based systems for detection of Cronobacter species

Fricker-Feer, C; Cernela, N; Bolzan, S; Lehner, Angelika; Stephan, Roger (2011). Evaluation of three commercially available real-time PCR based systems for detection of Cronobacter species. International Journal of Food Microbiology, 146(2):200-202.

Abstract

In the last few years, various PCR based methods have been developed that enable detection of Cronobacter spp. to the genus and species level. Moreover, several real-time PCR based systems for detection of Cronobacter spp. are available, however, comparative evaluation studies are not available. The current study represents a comparative evaluation of three commercial diagnostic systems, namely the BAX® System PCR Assay Enterobacter sakazakii (DuPont, Qualicon, Wilmington, USA), the Assurance GDS™ Enterobacter sakazakii (BioControl, Bellvue, USA) and the foodproof® Enterobacter sakazakii Detection Kit (Biotecon Diagnostics, Potsdam, Germany) for the rapid identification of Cronobacter spp. Twenty-one target and non-target strains were included in the study and results were compared for specificity and convenience in performance. A specificity of 100% was observed for two of the three real time PCR systems tested, namely the Assurance GDS™ Enterobacter sakazakii and the foodproof® Enterobacter sakazakii Detection Kit for pure cultures as well as artificially contaminated powdered infant formula (PIF) samples.

Copyright © 2011 Elsevier B.V. All rights reserved.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinärwissenschaftliches Institut > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > Food Science
Life Sciences > Microbiology
Language:English
Date:2011
Deposited On:17 Mar 2012 17:24
Last Modified:07 Nov 2024 02:37
Publisher:Elsevier
ISSN:0168-1605
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.ijfoodmicro.2011.02.006
PubMed ID:21382642
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