The ampliﬁed fragment length polymorphism (AFLP) technique is a widely used multi-purpose DNA ﬁngerprinting tool. The ability to size-separate ﬂuorescently labelled AFLP fragments on a capillary electrophoresis instrument has provided a means for high-throughput genome screening, an approach particularly useful in studying the molecular ecology of non-model organisms. While the ‘per-marker-generated’ costs for AFLP are low, ﬂuorescently labelled oligonucleotides remain costly. We present a cost-effective method for ﬂuorescently end-labelling AFLPs that should make this tool more readily accessible for laboratories with limited budgets. Both standard ﬂuorescent AFLPs and the end-labelled alternatives pre- sented here are repeatable and produce similar numbers of fragments when scored using both manual and automated scoring methods. While it is not recommended to combine data using the two approaches, the results of the methods are qualitatively comparable, indicating that AFLP end-labelling is a robust alternative to standard methods of AFLP genotyping. For researchers commencing a new AFLP project, the AFLP end-labelling method outlined here is easily implemented, as it does not require major changes to PCR protocols and can signiﬁcantly reduce the costs of AFLP studies.