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Structural and physiological phenotypes of disease-linked lamin mutations in C. elegans

Bank, Erin M; Ben-Harush, Kfir; Feinstein, Naomi; Medalia, Ohad; Gruenbaum, Yosef (2012). Structural and physiological phenotypes of disease-linked lamin mutations in C. elegans. Journal of Structural Biology, 177(1):106-112.

Abstract

The nuclear lamina is a major structural element of the nucleus and is predominately composed of the intermediate filament lamin proteins. Missense mutations in the human lamins A/C cause a family of laminopathic diseases, with no known mechanistic link between the position of the mutation and the resulting disease phenotypes. The Caenorhabditis elegans lamin (Ce-lamin) is structurally and functionally homologous to human lamins, and recent advances have allowed detailed structural analysis of Ce-lamin filaments both in vitro and in vivo. Here, we studied the effect of laminopathic mutations on Ce-lamin filament assembly in vitro and the corresponding physiological phenotypes in animals. We focused on three disease-linked mutations, Q159K, T164P, and L535P, which have previously been shown to affect lamin structure and nuclear localization. Mutations prevented the proper assembly of Ce-lamin into filament and/or paracrystalline arrays. Disease-like phenotypes were observed in strains expressing low levels of these mutant lamins, including decreased fertility and motility coincident with muscle lesions. In addition, the Q159K- and T164P-expressing strains showed a reduced lifespan. Thus, different disease-linked mutations in Ce-lamin exhibit major effects in vivo and in vitro. Using C. elegans as a model system, a comprehensive analysis of the effects of specific lamin mutations from the level of in vitro filament assembly to the physiology of the organism will help uncover the mechanistic differences between these different lamin mutations.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Structural Biology
Language:English
Date:2012
Deposited On:30 Oct 2012 16:19
Last Modified:22 Oct 2024 03:38
Publisher:Elsevier
ISSN:1047-8477
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.jsb.2011.10.009
PubMed ID:22079399

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