This study evaluated between-sample memory in isotopic measurements of δ2H and δ18O in water samples by laser spectroscopy. Ten isotopically depleted water samples spanning a broad range of oxygen and hydrogen isotopic compositions were measured by three generations of off-axis integrated cavity output spectroscopy and cavity ringdown spectroscopy instruments. The analysis procedure encompassed small (less than 2 ‰ for δ2H and 1 ‰ for δ18O) and large (up to 201‰ for δ2H and 25‰ for δ18O) differences in isotopic compositions between adjacent sample vials. Samples were injected 18 times each, and the between-sample memory effect was quantified for each analysis run. Results showed that samples adversely affected by between-sample isotopic differences stabilised after seven–eight injections. The between-sample memory effect ranged from 14 % and 9% for δ2H and δ18O measurements, respectively, but declined to negligible carryover (between 0.1 % and 0.3 % for both isotopes) when the first ten injections of each sample were discarded. The measurement variability (range and standard deviation) was strongly dependent on the isotopic difference between adjacent vials. Standard deviations were up to 7.5 ‰ for δ2H and 0.54 ‰ for δ18O when all injections were retained in the computation of the reportable δ-value, but a significant increase in measurement precision (standard deviation in the range 0.1 ‰–1.0 ‰ for δ2H and 0.05 ‰–0.17 ‰ for δ18O) was obtained when the first eight injections were discarded. In conclusion, this study provided a practical solution to mitigate between-sample memory effects in the isotopic analysis of water samples by laser spectroscopy.