In three Bavarian populations of Alytes obstetricans that were studied for the occurrence of Batrachochytrium dendrobatidis, the pathogen was detected. This is the first account of chytridiomycosis in Bavaria, Germany. Infected tadpoles had low infection loads mostly of 101 to 102 genome equivalents. Under high-density rearing conditions in the laboratory mortality rates were high after metamorphosis. Some individuals, however, showed no infection with Bd, while others survived metamorphosis in spite of low Bd loads. A new approach was chosen to get historical data on Bd occurrence in one of these populations: skeletochronological phalanx cross sections of 248 individuals that had been collected in the late
1980s were used to analyse the epidermis for chytrid sporangia. No sporangia were detected, thus we conclude that this population was not affected by Bd in the past.