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Studying nonobstructive azoospermia in cystinosis: histologic examination of testes and epididymis and sperm analysis in a Ctns⁻/⁻ mouse model


Besouw, Martine T P; van Pelt, Ans M M; Gaide Chevronnay, Héloïse P; Courtoy, Pierre J; Pastore, Anna; Goossens, Ellen; Devuyst, Olivier; Antignac, Corinne; Levtchenko, Elena N (2012). Studying nonobstructive azoospermia in cystinosis: histologic examination of testes and epididymis and sperm analysis in a Ctns⁻/⁻ mouse model. Fertility and Sterility, 98(1):162-165.

Abstract

OBJECTIVE: To study the pathogenesis of male infertility in cystinosis due to nonobstructive azoospermia, using a Ctns(-/-) mouse model. DESIGN: Observational case-control study. SETTING: Academic research laboratory. ANIMAL(S): Male C57BL/6 Ctns(-/-) mice were compared with C57BL/6 wild-type (wt) mice. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Fertility was studied using litter size (n = 3 vs. n = 2). After animals were sacrificed, testes, epididymis, and vas deferens were removed for testicular cystine measurements (n = 5 vs. n = 6), histologic studies (n = 3 vs. n = 3), and sperm analysis (n = 3 vs. n = 3). RESULT(S): Mean testicular cystine content was significantly higher in Ctns(-/-) mice compared with wt mice (26.6 ± 1.22 vs. 0.1 ± 0.01 nmol cystine/mg protein). Testes of Ctns(-/-) mice had lower weight compared with wt mice (0.096 ± 0.009 g vs. 0.112 ± 0.004 g), but mice fertility was similar (litter size 6.6 ± 1.4 vs. 6.3 ± 2.6 pups). Neither histologic nor sperm abnormalities were found. CONCLUSION(S): The Ctns(-/-) mouse model generated on C57BL/6 background is not suitable for clarifying the pathogenesis of male infertility in cystinosis. The etiology of nonobstructive azoospermia in these patients remains unclear.

Abstract

OBJECTIVE: To study the pathogenesis of male infertility in cystinosis due to nonobstructive azoospermia, using a Ctns(-/-) mouse model. DESIGN: Observational case-control study. SETTING: Academic research laboratory. ANIMAL(S): Male C57BL/6 Ctns(-/-) mice were compared with C57BL/6 wild-type (wt) mice. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Fertility was studied using litter size (n = 3 vs. n = 2). After animals were sacrificed, testes, epididymis, and vas deferens were removed for testicular cystine measurements (n = 5 vs. n = 6), histologic studies (n = 3 vs. n = 3), and sperm analysis (n = 3 vs. n = 3). RESULT(S): Mean testicular cystine content was significantly higher in Ctns(-/-) mice compared with wt mice (26.6 ± 1.22 vs. 0.1 ± 0.01 nmol cystine/mg protein). Testes of Ctns(-/-) mice had lower weight compared with wt mice (0.096 ± 0.009 g vs. 0.112 ± 0.004 g), but mice fertility was similar (litter size 6.6 ± 1.4 vs. 6.3 ± 2.6 pups). Neither histologic nor sperm abnormalities were found. CONCLUSION(S): The Ctns(-/-) mouse model generated on C57BL/6 background is not suitable for clarifying the pathogenesis of male infertility in cystinosis. The etiology of nonobstructive azoospermia in these patients remains unclear.

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Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology

04 Faculty of Medicine > Center for Integrative Human Physiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Health Sciences > Reproductive Medicine
Health Sciences > Obstetrics and Gynecology
Language:English
Date:2012
Deposited On:24 Jan 2013 12:35
Last Modified:30 Jul 2020 07:04
Publisher:Elsevier
ISSN:0015-0282
OA Status:Green
Publisher DOI:https://doi.org/10.1016/j.fertnstert.2012.03.050
PubMed ID:22578532

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