Navigation auf zora.uzh.ch

Search ZORA

ZORA (Zurich Open Repository and Archive)

DDOS: due to massive botnet requests against our ‘Advanced Search’ we have restricted access to UZH (local and VPN). Thank you for your understanding.

Identification of Burkholderia cenocepacia Strain H111 virulence factors using nonmammalian infection hosts

Schwager, S; Agnoli, K; Kothe, M; Feldmann, F; Givskov, M; Carlier, A; Eberl, L (2013). Identification of Burkholderia cenocepacia Strain H111 virulence factors using nonmammalian infection hosts. Infection and Immunity, 81(1):143-153.

Abstract

Burkholderia cenocepacia H111, a strain isolated from a cystic fibrosis patient, has been shown to effectively kill the nematode Caenorhabditis elegans. We used the C. elegans model of infection to screen a mini-Tn5 mutant library of B. cenocepacia H111 for attenuated virulence. Of the approximately 5,500 B. cenocepacia H111 random mini-Tn5 insertion mutants that were screened, 22 showed attenuated virulence in C. elegans. Except for the quorum-sensing regulator cepR, none of the mutated genes coded for the biosynthesis of classical virulence factors such as extracellular proteases or siderophores. Instead, the mutants contained insertions in metabolic and regulatory genes. Mutants attenuated in virulence in the C. elegans infection model were also tested in the Drosophila melanogaster pricking model, and those also attenuated in this model were further tested in Galleria mellonella. Six of the 22 mutants were attenuated in D. melanogaster, and five of these were less pathogenic in the G. mellonella model. We show that genes encoding enzymes of the purine, pyrimidine, and shikimate biosynthesis pathways are critical for virulence in multiple host models of infection.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
Dewey Decimal Classification:580 Plants (Botany)
Scopus Subject Areas:Life Sciences > Parasitology
Life Sciences > Microbiology
Life Sciences > Immunology
Health Sciences > Infectious Diseases
Language:English
Date:2013
Deposited On:21 Mar 2013 16:02
Last Modified:08 Jun 2025 01:37
Publisher:American Society for Microbiology
ISSN:0019-9567
OA Status:Closed
Publisher DOI:https://doi.org/10.1128/IAI.00768-12
PubMed ID:23090963
Full text not available from this repository.

Metadata Export

Statistics

Citations

Dimensions.ai Metrics
38 citations in Web of Science®
41 citations in Scopus®
Google Scholar™

Altmetrics

Authors, Affiliations, Collaborations

Similar Publications