PURPOSE: Dynamic contour tonometry (DCT) is a new technique to measure intraocular pressure (IOP). In several studies no correlation between IOP values and corneal thickness was shown with DCT. This is in contrast to the gold standard, Goldmann applanation tonometry (GAT). The authors evaluated the reproducibility (RP) of DCT compared to GAT, a prerequisite for its introduction into clinical routine. METHODS: IOP was measured with both DCT and GAT in 50 subjects with normal cornea. To evaluate the short-term RP, four DCT and four GAT measurements were performed at day 1 in a randomized order. Long-term RP was determined by one additional measurement per method at day 2, 5, and 8. RESULTS: The short-term RP was defined as the mean value of all standard deviations (SD) of the individual measurements at day 1. Short-term RP was 1.1 mmHg for GAT and 1.2 mmHg for DCT. For long-term reproducibility, mean SD was 1.2 mmHg for GAT and 1.5 mmHg for DCT. Bland-Altman revealed a good agreement of the two methods. However, mean DCT values were on average 0.8+/-1.1 mmHg higher than GAT values. A significant correlation was observed between GAT and CCT (r2=0.15, p=0.006), but not between DCT and CCT (r2=0.032, p=0.21). CONCLUSIONS: Short- and long-term reproducibility of DCT is comparable to that of GAT. GAT is more affected by CCT than DCT, measuring higher IOPs in eyes with higher central corneal thickness.